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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 1 / Published : 2014-02

TOC : 7, and page :43 - 48

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Original Article :

Effect of seminal plasm of cow on goat’s spermatozoa plasma membran in milk diluter on semen freezhing

Author :

  1. Suherni Susilowati*1
  1. Dosen Fakultas Kedokteran Hewan

Abstract :

Up till now semen freezing process of ram’s spermatozoa has not yet satisfying, this is because ram’s seminal plasma contain phospholipase A from bulbourethral gland, that can coagulate with egg yolk in diluter. Because of that researchers want to replace ram’s seminal plasma with bull’s seminal plasma in milk diluter media. Special aim of this research is to understand the benefit of bull’s seminal plasma in diluter of ram’s spermatozoa. General purpose of this research is using bull’s seminal plasma in diluter of ram’s spermatozoa to improve fresh semen quality and even frozen semen. This research consists of three treatments. Control treatment (PO) are diluter + ram’s semen (without seminal plasma). Treatment I (PI) are diluter + spermatozoa + bull’s seminal plasma (1:1), treatment II (PII) are diluter + spermatozoa + bull’s seminal plasma (1:2). All of treatments observed everyday of motility, viability, plasma membran integrity and spermatozoa’s DNA integrity. Observation in diluter without seminal plasma shows lowest spermatozoa’s membrane plasma integrity in day I, II, III, IV, and V. Anova test for spermatozoa’s membrane plasma integrity shows significant different (p <0,05) between PO, PI, PII, and PIII. Duncan's multiple range test for observation PI group produce highest spermatozoa’s membrane plasma integrity percentage.In conclusion, addition of bull’s seminal plasma can mantain, membrane plasma integrity in skim milk diluter

Keyword :

Seminal plasma, Bull, ram’s, Membran plasma integrity,

References :

  1. Beatriz, B, Perez-Pe, A. Gallego Tato, J. Osada, T. Muino Blanco and J.A.Cebrian Perez, (2000). Seminal Plasma Protein Revert the Cold Shock Damage on Ram Sperm Membrane. . : Biology Reproduction. 63:1531-1537
  2. Lamirande, E.H., A. Jiang, H. Kodana, and C. Gagnon, (1997). Reactive Oxygen Species and Sperm Physiology. . : Rev.Reprod. 2(1) :48-54
  3. Ramukhiti, F, L. N. Tshimangadzo, B. Sutherland and C.L. Khoboso, (2011). Cryopreservation of South African indgenous goat semen. African Journal of Biotechnology. Africa : African Journal of Biotechnology. 10 (77): 17898-17901


Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2014-02
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  2. Effect of infusum salam leaves (eugenia polyantha) on blood glucose level on the rat (rattus norwegicus) in alloxan induced
  3. Potential of phytase ezymes from rumen bacteria on sem analysis structural changes of rice bran as broiler feed
  4. The comparison of morphology between bovine oocyte vitrification before and after in vitro maturation
  5. The effect of exposing pregnant rat (rattus novergicus) to particulate matter on congenital disorder and resorption of embryos
  6. Ha gene phyloanalysis of influenza h1n1 2009 virus in surabaya
  7. Effect of seminal plasm of cow on goat’s spermatozoa plasma membran in milk diluter on semen freezhing
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