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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

TOC : 4, and page :15 - 18

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Original Article :

Erythrocyte's form changes in dog's blood smear before and after the storage using citrate phosphate dextrose

Author :

  1. Soepraptini, Julien*1
  2. Widyayanti, Kristina *2
  3. Estoepangestie, A.T.S.*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of the study was to determine the effect of Citrate Phosphate Dextrose (CPD) anticoagulant for the preservation of dog's blood. Sample used in this study was blood of 1,5 to 2 years old 9 healthy male dogs with 2,8 ml CPD anticoagulant and mixed well. Blood sample was keep in refrigerator of 4 C for 21 days. Blood smear was madebefore and after storage to detect the abnormality of erythrocyte, such as crenation and rouleaux form. Pre and post test design was used in this study, and the data was analysed by paired t-test. The result of the study showed no significanly difference (p>0.05) of erythrocyte abnormality before and after storage. This result indicates CPD anticoagulant used in dog's blood preservation doesn't impact to the abnormality of erythrocyte.

Keyword :

Citrate Phosphate Dextrose, Blood Storage, Blood Smear, Crenation, .,


References :

  1. DeNicola, D.B, (2006). Hematologic Evaluation of Dog and Cats II : Erythrocyte Morphology : Clues to the Cause of Disease. Maine : IDEXX Laboratories, Inc.
  2. Colville, J., (2002). Blood, Lymph, and Immunity. Chap. 9, In : T. Colville, and J.M. Bassert. Clinical Anatomy and Physiology For Ve t e r i na r y Te c hni c i a ns . Missouri : Mosby, Inc
  3. Battaglia, A.M, (2001). Small Animal Transfusion Medicine. Chap. 5, In: A.M. Battaglia. Small Animal Emergency and Critical Care : A Manual For Veterinary Technicians. United States : W.B. Saunders Company


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
  1. Production and specificity testing of monoclonal antibodies to bovine zonna pellucida 3 deglycosylated (mab-bzp3dg) for woman immunocontraceptive vaccine
  2. Potency of crude spirulina on protein efficiency ratio in laying hen
  3. H-y antisera preparation and x chromosomal receptor tracer as sex determination prototype
  4. Erythrocyte's form changes in dog's blood smear before and after the storage using citrate phosphate dextrose
  5. Avian influenza h5n1 vaccine candidate for chicken from east java isolate virus
  6. Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c
  7. Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture
  8. Respon hmg toward ovarium development in goat
  9. Identification of pregnancy associated glycoprotein (pag) from milk of pregnant dairy cattle
  10. The role of soil as a helminths transmitter around the habitas of babirusa
  11. Potency of brown seaweed (sargassum duplicatum bory) ethanol and ethyl acetic fraction to malondialdehyde concentration decreasing and histological retriveal of ibd (inflammatory bowel disease) rat small intestinal jejunum
  12. Blood glucose and total blood protein profile in sheep provided with lactic acid bacteria and yeast on king grass and rice straw
  13. Effect of pegagan (centella asiatica) extract in ovariectomized wistar-strain rattus norvegicus on epithelial proliferation of vaginal wall
  14. Excessive dose of vitamine a on skeletal development in the mice embryos