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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

TOC : 7, and page :37 - 42

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Original Article :

Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture

Author :

  1. Lamid, Mirni *1
  2. Nugroho, T.P*2
  3. Chusniati, Sri *3
  4. Rochiman, Kusriningrum *4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This research was conducted to isolation and identify bacterium of cellulolytic of rumen liquid beef cattle by using media of Carboxil Methile Cellulose (CMC). Intake of sample done by three times with accomodating dilution of rumen beef cattle, packed into the bottle. After that was done by thinning 10, then taken by 1 ml was inoculated to containing of petridish with CMC using pour plate method for three days incubation. Identification to be conducted by macroskopic and microscopic which continued with biochemical test with consist of Triple Sugar Iron (TSIA),Sulfide Indol Motility (SIM), Manitol, Citrat, Urea and Glucose Test (Lactose, Dextrose, Sucrose, Saccarosa andmaltose). The result of research obtained by  seven bacterium species consist of: Nitrosomonas europae, Bacillussphaericus, Cellulomonas cellulans, Cytophaga hutchinsoi, Acidothermus cellulyticus, Lactobacillus acidophilus, dan Cellvibrio mixtus. All of this cellulolytic bacterium had potential to be used fermented waste agriculture for feed of ruminants.

Keyword :

Bacterium of cellulolytic, Carboxil Methile Cellulose, Waste Agriculture,


References :

  1. Aklyosov, (2004). Cellulase Enzyme That Degrades Celulose From Fungi. United States : Application of Microbial
  2. Atlas, M. R., (1988). Microbiologi Fundamental and Aplications. New York : Macmillan Publishing Company
  3. Alexander M., , (1976). Introduction to Soil Microbiology. New York : Jhon & Sons


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
  1. Production and specificity testing of monoclonal antibodies to bovine zonna pellucida 3 deglycosylated (mab-bzp3dg) for woman immunocontraceptive vaccine
  2. Potency of crude spirulina on protein efficiency ratio in laying hen
  3. H-y antisera preparation and x chromosomal receptor tracer as sex determination prototype
  4. Erythrocyte's form changes in dog's blood smear before and after the storage using citrate phosphate dextrose
  5. Avian influenza h5n1 vaccine candidate for chicken from east java isolate virus
  6. Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c
  7. Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture
  8. Respon hmg toward ovarium development in goat
  9. Identification of pregnancy associated glycoprotein (pag) from milk of pregnant dairy cattle
  10. The role of soil as a helminths transmitter around the habitas of babirusa
  11. Potency of brown seaweed (sargassum duplicatum bory) ethanol and ethyl acetic fraction to malondialdehyde concentration decreasing and histological retriveal of ibd (inflammatory bowel disease) rat small intestinal jejunum
  12. Blood glucose and total blood protein profile in sheep provided with lactic acid bacteria and yeast on king grass and rice straw
  13. Effect of pegagan (centella asiatica) extract in ovariectomized wistar-strain rattus norvegicus on epithelial proliferation of vaginal wall
  14. Excessive dose of vitamine a on skeletal development in the mice embryos