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Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 3 / Published : 2010-11

TOC : 14, and page :221 - 230

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Original Article :

Expression of adhf-36kda protein towards osmolarity and ph in vitro environmental of salmonella typhi (as study to adhesin molecule-based candidate vaccine for typhoid fever)

Author :

  1. I Nengah Kundera*1
  2. Sanarto Santoso*2
  3. Aulanni’am*3
  4. Sri Winarsih*4
  1. FMIPA Universitas Tadulako
  2. FMIPA Universitas Brawijaya
  3. FMIPA Universitas Brawijaya
  4. FMIPA Universitas Brawijaya

Abstract :

Salmonella Typhi is a gram-negative pathogen cause of typhoid fever disease, and continous to be distressing health problem worldwide. The outbreak infection deseases to spread very related of virulence factor, pathogensis, and ability adaptation of Salmonella Typhi and live at animal, human bodys and environmental. AdhF-36kDa protein is a subunit protein to considered responsible for the adhesion and colonization factors of Salmonella Typhi.This research aims to determine the expression of AdhF-36kDa protein for osmolarity and pH in vitro environmental of Salmonella Typhi, as study to adhesin molecule-based candidate vaccine for typhoid fever. Strains used in this research originated from patients with typhoid fever in the Saiful Anwar hospital-Malang. The results showed stability expression of AdhF-36kDa protein on exposure to osmolarity, and tends to increase until at a concentration of 350mM NaCl. However, different treatment and pH changes in protein AdhF-36kDa expression patterns from pH4,5 to 6,0 because of the result in bend profiles SDS-PAGE protein increased concentration thinner pH. This proves that the change environmental signals affect the virulence factor of Salmonella Typhi, which is regulated by genes fimYZ. Therefore AdhF-36kDa protein, as known subunit protein fimbriae type-1 as potential developed vaccine candidate, because expression this protein stable when the environment signal changes.

Keyword :

AdhF-36kDa protein, Salmonella Typhi, osmolarity, pH, .,

References :

  1. Arricau, N., D.Herman., H.Waxin., C.Ecobichon., P.S.Duffey., and M.Y.Popoff, (1998). The RcsB-RcsC regulatory system of Salmonella Typhi differentially modulates the expression of invasion protein, flagellin and Vi antigen in response to osmolarity. . : Mol.Microbiol
  2. Brett-Finlay and Siebers, (1995). Mechanisms of Mucosal Colonization and Penetration by Bacterial Pathogens ; in Virulence Mechanisms of Bacterial Pathogens. Washington : ASM Press
  3. Bucarey, S. A., N.A. Villagra., M.P. Martinic., A. N.Trombert., C.A. Santiviago., N. P. Maule´n., P, (2005). The Salmonella enterica Serovar Typhi tsx Gene, Encoding a Nucleoside-Specific Porin, Is Essential for Prototrophic Growth in the Absence of Nucleosides. . : Infect. Immun


Archive Article

Cover Media Content

Volume : 3 / No. : 3 / Pub. : 2010-11
  1. Garlic as an alternative disinfectant to the escherichia coli
  2. Centrifugation of goat semen at freezing process with some concentrate of egg yolk diluent to the quality of sperm
  3. Influence of the avian influenza virus subtype h5n1 infection toward sperm motility and life in long tail monkey (macaca fascicularis)
  4. Vfa concentration and acetate, propionate, butyrate molar proporsions rumen of ongole grade cattle with ammoniation rice straw, soybean straw and rice straw
  5. The activity of achyranthes aspera linn alkaloid to cause aopoptotic and dna fragmentation on breast cancer
  6. The antimitogenic effect of alkaloid achyranthes aspera linn on apoptotic induction in cell infection by mycobacterium tuberculosis
  7. Stages of vaccination and the type of vaccine used to prevent infectious disease in chickens
  8. Effect of complex of insulin like growth factor-1 and insulin like growth factor binding protein-3 on the increase number of ovarian follicle in female mice (mus musculus)
  9. Spleen weight and white pulp diameter picture in broiler chickens exposed to cronic heat stress
  10. Identification of kit-ligand from in vitro maturation of bovine oocytes with electrophoresis method
  11. Cellulolityc bacteria for increasing quality of complete feed from waste product
  12. Identification of cyclin-dependent kinase (cdk1) that express on bovine cumulus oocyte complex after a model oocyte maturation experimence
  13. Utilization of bakteriocin in the handling of sub clinical mastitis and the effect on milk quality on the number of bacteria
  14. Expression of adhf-36kda protein towards osmolarity and ph in vitro environmental of salmonella typhi (as study to adhesin molecule-based candidate vaccine for typhoid fever)
  15. Motility and live percentage test in ram’s spermatozoa in isotonic commercial liquid and egg yolk assortment extender
  16. Identification of the gene for staphylococcus aureus toxic shock syndrome toxin-1 (tsst-1) from bovine mastitis milk