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Veterinaria Medika

ISSN 1979-1305

Vol. 2 / No. 1 / Published : 2009-02

TOC : 6, and page :29 - 34

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Original Article :

Glycoprotein of human membrane spermatozoa

Author :

  1. Amy Tenzer*1
  2. Umie Lestari*2
  1. Fakultas MIPA Jurusan Biologi Universitas Negeri Malang
  2. Fakultas MIPA Jurusan Biologi Universitas Negeri Malang

Abstract :

The contain protein in mammal, especially, human sperm membrane is , 50%, that distributed astrans membrane protein. The protein membrane of human sperm have any function as transport system,protein carrier and receptor or ligand and have numerous antigenic determinants including carbohydrate and protein, namely glycoprotein, that can be corfirmed by PAS detection. This study focused on the identification of proteins membrane from human sperm based on its ratio of protein, carbohydrate and total glycoprotein. Confirmation of protein concentration measured Biuret method and the ratio glycoprotein;carbohydrate and protein measured by Periodic Acid Schiff (PAS) reagent, which detected aldehyde groupin sugar produced by action of periodic acid. The molecular weight of glycoprotein membrane of humanspermatozoa was detected by showing through the use of SDS-PAGE. The result showed that protein band with molecular weight of 97, 32 and 27 kDa that contained glycoprotein 25,52; 28,91; 26,17 mg/ml,carbohydrate of 10,84; 11,97 and 10,57 mg/ml and protein of 14.96; 16,94 and 15.34 mg/ml, respectively.It can be concluded that the ratio glycoprotein; carbohydrate and protein of human sperm membrane are 2:1:1; 2:1 :1 and 2.5:1:1,6, for glycoprotein with molecular weight of 97, 32 and 27 kDa, respectively.

Keyword :

Glycoprotein, human sperm membrane, ., ., .,


References :

  1. Yanagimachi,R. , (1998). Mammalian Fertilization. In The Physiology Of Reproductive . New York : Raven Press, Ltd.
  2. Schroter S, Osterhoff C, Mc Ardle W., Ivell R., (1999). The Glycocalyx of The Sperm surface. United States : Hum Reprod Update


   


Archive Article

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Volume : 2 / No. : 1 / Pub. : 2009-02
  1. The effect of water extract from phyllanthus niruri l. is immunostimulator in newcastle disease vaccinated chicken
  2. Proliferation activity of gland mammae after leaves gynura procumbens extract which dmba (dimethylbenz(a)antrasen) initiation on sprague dawley rat
  3. Growth differentiation factor-9 (gdf-9) characterization on bovine oocyte collected from preanthral follicle using dotblotting method
  4. Optical density value of antibody against pregnancy-associated glycoproteins (pags) from the pregnant dairy cow blood serum in male local rabbit (oryctolagus cuniculus)
  5. Implementation studies of artificial insemination program of beef cattle in west java
  6. Glycoprotein of human membrane spermatozoa
  7. Specifitacy test anti-prostaglandin f2a indirect elisa method
  8. The sensitivity test of some antimicrobial agents to staphylococcus aureus of cause mastitis at dairy cattle at around of suka makmur dairy cooperative grati pasuruan
  9. Correlation between subclinical mastitis stages use california mastitis test with total bacteria in milk
  10. Detection of progesterone of pregnant dairy cattle using enzyme immunoassay (eia) method
  11. Identification of encoding gene for fibrinogen binding protein on staphylococcus aureus in mastitic milk
  12. The polymorphism of coagulase gene as searching strain variability of staphylococcus aureus from bovine mastitis cases
  13. Concentration of total protein from goat seminal plasma by biuret method
  14. The consumption of crude protein of haylage complete feed on ongole crossbreed steers
  15. Crude protein contents and dry matter in ipome sp fermentation as alternative for feed stuff
  16. The effect of forced moulting in the avian as causes of zoonocis case salmonella enteridis (se)