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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

TOC : 3, and page :9 - 14

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Original Article :

H-y antisera preparation and x chromosomal receptor tracer as sex determination prototype

Author :

  1. Anwar, Husni*1
  2. Srianto, Pudji*2
  3. Yuniarti, W.M*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This research to purposed that unweaning mice testes and lien synthesized H-Y protein, and then to knew that  polyclonal antibody H-Y could synthesized by male rabbit with immunization process. Biological test also usedat laboratory animal, that protein H-Y coud displacement ratio sex.This research used laboratory animals like, unweaning mice, male rabbit and male and female mice, usingimmunization method and blotting and immunohistochemistry techniques to produced and proved H-Y antigen. Result of this research are, testes unweaning mice produced H-Y protein; polyclonal antibody H-Y could produced from male rabbit by immunization process; immunohistochemistry techniques could known that H-Y protein expressed in seminiferus tubule and injection of H-Y antigen in male mice could displaced sex ratio. Conclusions of this research are 1). Testes unweaning mice produced H-Y protein, 2). Polyclonal antibody could produced by immunized male rabbit, 3). Immunohistochemistry techniques could known expressed of H-Yprotein and 4). Protein H-Y could displaced sex ratio

Keyword :

H-Y protein, Immunization, Immunohistochemistry, Polyclonal antibody, Dot Blot,


References :

  1. Anzar,M.,He., L., M.M.Buhr, T.G. Kroetsch and K.P. Pauls, (2002). Sperm Apoptosis in Fresh and Cryopreserved Bull Semen Detected by Flow Cytometry and It's Relationship with Fertility. United States : J. Biol. Reprod
  2. Bearden,H.J. and J.W.Fuquay, (1992). Applied Animal Reproduction. New Jersey : Pretince Hall Englewood Cliffs
  3. Hafez,E.S.E, (2000). Reproduction in Farm Animals 7th edition. Philladelphia : Lea & Febiger


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
  1. Production and specificity testing of monoclonal antibodies to bovine zonna pellucida 3 deglycosylated (mab-bzp3dg) for woman immunocontraceptive vaccine
  2. Potency of crude spirulina on protein efficiency ratio in laying hen
  3. H-y antisera preparation and x chromosomal receptor tracer as sex determination prototype
  4. Erythrocyte's form changes in dog's blood smear before and after the storage using citrate phosphate dextrose
  5. Avian influenza h5n1 vaccine candidate for chicken from east java isolate virus
  6. Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c
  7. Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture
  8. Respon hmg toward ovarium development in goat
  9. Identification of pregnancy associated glycoprotein (pag) from milk of pregnant dairy cattle
  10. The role of soil as a helminths transmitter around the habitas of babirusa
  11. Potency of brown seaweed (sargassum duplicatum bory) ethanol and ethyl acetic fraction to malondialdehyde concentration decreasing and histological retriveal of ibd (inflammatory bowel disease) rat small intestinal jejunum
  12. Blood glucose and total blood protein profile in sheep provided with lactic acid bacteria and yeast on king grass and rice straw
  13. Effect of pegagan (centella asiatica) extract in ovariectomized wistar-strain rattus norvegicus on epithelial proliferation of vaginal wall
  14. Excessive dose of vitamine a on skeletal development in the mice embryos