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Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 1 / Published : 2008-02

TOC : 8, and page :39 - 42

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Original Article :

Identification of excretion-secretion protein profile of the adult haemonchus contortus with sds-page

Author :

  1. Nunuk Dyah Retno Lastuti*1
  2. Artha Rini Pasila*2
  3. Tri Nurhayati*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this research is to identify excretion – secretion protein (ESP) profile of Haemonchus contortuswhich presented in mollecular weight. One hundred female Haemonchus contortus were isolated from sheepsand goats’ abomasum from Surabaya Slaughter House, worms were washed by Phosphat Buffer Saline (PBS)then incubated in PBS with pH 7,0 and temperature 37°C for a night. Excretion – secretion liquid that wormsproduced in PBS isolated with saturated ammonium, then SDS-PAGE (Sodium Dodecyl Sulphate-PolyacrylamidGel Electrophorese) method used to identified excretion – secretion protein profile. Result of this research got5 excretion – secretion protein profile : 42,3 kilo Dalton (kDa); 39,3 kDa; 28,9 kDa; 24,4 kDa and 13,9 kDa.

Keyword :

Haemonchus contortus, ESP, SDS-PAGE, ., .,


References :

  1. Artama, Wayan. T., (1991). Rekayasa Genetika. Yogyakarta : PAU – Bioteknologi Universitas Gajah Mada
  2. Koswara, O., (1998). Peran Serta Masyarakat Dalam Upaya Pengendalian Penyakit Parasitik Pada Hewan. Prosiding Seminar Parasitologi Nasional IV. Jakarta : Perkumpulan Pemberantasan Penyakit Parasit Indonesia


   


Archive Article

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Volume : 1 / No. : 1 / Pub. : 2008-02
  1. Identification of staphylococcus aureus which causes mastitis with mannitol test and detection of acetoin product in dairy cattke at the farm area of suka makmur dairy cooperative grati pasuruan
  2. The influence of api - api (avicennia marina) leaves extract toward embryo resorbtion, body weight and lengh toetal mice (mus musculus)
  3. The effects of eimeria tenella infection on the spleen weight, size and white pulp diameters in the chickens primary infection and secondary infection
  4. Detection of avian influenza virus subtype h5 on stray cats (felis silvestris catus) in bandung city area
  5. The effect of alkaloid fraction of jarong (achyrantes aspera linn) on viability culture myeloma cell mice
  6. Antibacterial activity of the ethanol extract pluchea indica less leaves against escherichia coli by in vitro
  7. Identification of protein profile of sarcoptes scabiei on goats by sds-page analysis
  8. Identification of excretion-secretion protein profile of the adult haemonchus contortus with sds-page
  9. Identification of aerob cellulolytic fungi from rumen fluid waste at pegirian slaughter house surabaya
  10. The effects of supplying commercial probiotic on the quantity and quality of dairy cow milk
  11. The effect of given crude chlorella to total cholesterol blood rates on broiler chicken
  12. The effect sgot and sgpt level of whiterat ( rattus norvegicus ) that was given juice of noni ( morinda citrifolia ) and high fatty diet
  13. Utility information technology ( it ) for head surface area of spermatozoa sheep with biometric measurement methode
  14. Influence of leaf extract of sirih ( piper betle l.) to growth staphylococcus aureus and escherichia coli with disk diffusion method
  15. Protein characterization of excretory secretory second stage larvae dorman against antibody anti-second stage larva toxocara canis by western blot techniques