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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

TOC : 9, and page :49 - 52

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Original Article :

Identification of pregnancy associated glycoprotein (pag) from milk of pregnant dairy cattle

Author :

  1. Samik, Abdul*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The sustainability of livestock sources is related to birth rate increase and the shortening of calving interval. One of the efforts to shorten the calving interval in dairy cattle is by conducting early diagnosis of pregnancy after mating. The development of early pregnancy diagnosis method in livestock can be undertaken by observing specific substance presents in the milk of pregnant livestock, i.e. the Pregnancy Associated Glycoprotein (PAG). The objective of this study was to isolate PAG protein from milk of pregnant dairy cattle as the base for developing one of early pregnancy test in cattle.This study was conducted by following these stages: 1) Isolation milk protein and 2) Identification of PAG protein from the milk of pregnant dairy cattle by SDS-PAGE and Western Blot method. The results of SDS-PAGE ofmilk pregnant dairy cattle PAG isolate revealed 10 protein bands with molecular weights between 37.85 and 210.51kDa. Specificity test with Western blot indicated protein band recognized by anti-PAG monoclonal antibody, believedt o be PAG protein with molecular weight of 66,41 kDa. Based on the results, it can be concluded that milk of pregnant dairy cattle express PAG protein with molecular weight 66,41 kDa.

Keyword :

Milk of dairy cattle, Milk protein, Pregnancy associated glycoprotein, ., .,


References :

  1. Sousa, N.M., B. Remy, B. El Amiri, J.R. de Figueiredo, H. Banga mboko, P. B. D. Goncalves a n d, (2002). Characterization of Pregnancy Associated Glycoprotein Extracted from Zebu (Bos indicus) Placental Removed at Different Gestational Periods. United States : Reprod.Nutr.Dev. 42, 227-241.
  2. El Amiri B, B Remy, NM De Sousa and JF Beckers., (2004). Isolation and characterization of eight pregnancy-associated glycoproteins present at hight level ovine placenta between day 60 and day 100 of gestation.. United States : Reprod. Nutr. Dev. 44:169-181
  3. Aulani'am, (2005). Protein & Analisisnya. Malang : Citra Mentari Goup


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
  1. Production and specificity testing of monoclonal antibodies to bovine zonna pellucida 3 deglycosylated (mab-bzp3dg) for woman immunocontraceptive vaccine
  2. Potency of crude spirulina on protein efficiency ratio in laying hen
  3. H-y antisera preparation and x chromosomal receptor tracer as sex determination prototype
  4. Erythrocyte's form changes in dog's blood smear before and after the storage using citrate phosphate dextrose
  5. Avian influenza h5n1 vaccine candidate for chicken from east java isolate virus
  6. Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c
  7. Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture
  8. Respon hmg toward ovarium development in goat
  9. Identification of pregnancy associated glycoprotein (pag) from milk of pregnant dairy cattle
  10. The role of soil as a helminths transmitter around the habitas of babirusa
  11. Potency of brown seaweed (sargassum duplicatum bory) ethanol and ethyl acetic fraction to malondialdehyde concentration decreasing and histological retriveal of ibd (inflammatory bowel disease) rat small intestinal jejunum
  12. Blood glucose and total blood protein profile in sheep provided with lactic acid bacteria and yeast on king grass and rice straw
  13. Effect of pegagan (centella asiatica) extract in ovariectomized wistar-strain rattus norvegicus on epithelial proliferation of vaginal wall
  14. Excessive dose of vitamine a on skeletal development in the mice embryos