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Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 1 / Published : 2008-02

TOC : 1, and page :1 - 8

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Original Article :

Identification of staphylococcus aureus which causes mastitis with mannitol test and detection of acetoin product in dairy cattke at the farm area of suka makmur dairy cooperative grati pasuruan

Author :

  1. Erni Rosilawati Sabar Iman*1
  2. L.P. Tirnata*2
  3. Anam Al-Arief*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aims of the study were to identify Staphylococcus aureus which causes mastitis in dairy cattle at thefarm area of Suka Makmur Dairy Cooperative by detecting the acetoin production with Voges-ProskauerTest comparing with mannitol fermentation test using Mannitol Salt Agar. The non probability samplingwas used on this research. Milk samples had been taken as much as 5-10 ml from each quarter ofdairy cattle which shown positive according to California Mastitis Test. Two hundred bovine milksamples were cultured on Blood Agar and MacConkey Agar from which 100 (50%) strains were identifiedas Staphylococcus sp. and 26 (13%) were identified as coagulase positive staphylococcus; which weresuspected as Staphylococcus aureus, whereas 25 strains of them showed the positive result of Voges-Proskauer Test and 24 strains showed the positive result of mannitol fermentation on Mannitol Salt Agarcultured. The results of the two methods were compared with Nonparametric Tests among which ChisquareTest were used. The result showed that there was no difference between the result of Voges-Proskauer Test and Mannitol Salt Agar cultured, so that Voges-Proskauer Test is able to replace MannitolSalt Agar cultured as the common identification method for Staphylococcus aureus.

Keyword :

Staphylococcus aureus, Mastitis, Mannitol Salt Agar, Acetoin Production, .,


References :

  1. Bannerman, D. D. and R. J. Wall, (2005). A Novel Strategy for the Prevention of Staphylococcus aureus-Induced Mastitis in Dairy Cows. Information Systems for Biotechnology News Report. Virginia, USA : Virginia Tech University
  2. Beishir, L., (1991). Microbiology in Practice: A Self- Instructional Laboratory Course. New York : HarperCollins Publishers Inc.


   


Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2008-02
  1. Identification of staphylococcus aureus which causes mastitis with mannitol test and detection of acetoin product in dairy cattke at the farm area of suka makmur dairy cooperative grati pasuruan
  2. The influence of api - api (avicennia marina) leaves extract toward embryo resorbtion, body weight and lengh toetal mice (mus musculus)
  3. The effects of eimeria tenella infection on the spleen weight, size and white pulp diameters in the chickens primary infection and secondary infection
  4. Detection of avian influenza virus subtype h5 on stray cats (felis silvestris catus) in bandung city area
  5. The effect of alkaloid fraction of jarong (achyrantes aspera linn) on viability culture myeloma cell mice
  6. Antibacterial activity of the ethanol extract pluchea indica less leaves against escherichia coli by in vitro
  7. Identification of protein profile of sarcoptes scabiei on goats by sds-page analysis
  8. Identification of excretion-secretion protein profile of the adult haemonchus contortus with sds-page
  9. Identification of aerob cellulolytic fungi from rumen fluid waste at pegirian slaughter house surabaya
  10. The effects of supplying commercial probiotic on the quantity and quality of dairy cow milk
  11. The effect of given crude chlorella to total cholesterol blood rates on broiler chicken
  12. The effect sgot and sgpt level of whiterat ( rattus norvegicus ) that was given juice of noni ( morinda citrifolia ) and high fatty diet
  13. Utility information technology ( it ) for head surface area of spermatozoa sheep with biometric measurement methode
  14. Influence of leaf extract of sirih ( piper betle l.) to growth staphylococcus aureus and escherichia coli with disk diffusion method
  15. Protein characterization of excretory secretory second stage larvae dorman against antibody anti-second stage larva toxocara canis by western blot techniques