Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 1 / Published : 2013-02

Order : 14, and page :75 - 78

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Original Article :

Identification pandemic h1n1-2009 virus on pigs in slaughterhouse pegirian surabaya by using hi test

Author :

  1. Prasetiyawati, Enny*1
  2. Nidom, C.A*2
  3. Sianita, Nanik*3
  4. Anwar, Chairul*4
  5. Ernawati, Rahayu*5
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga
  5. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this study was to identify the pandemic H1N1-2009 virus on pigs in Pegirian Surabaya slaughterhouse by using HI test. Nasal swab of pig sampels were taken from Pegirian Surabaya slaughterhouse. Before the swab samples were tested by HA test, they were inoculated to 810 days embryoned chicken eggs, then theywere incubated at 37 ºC for 3-4 days. On 3 day, the isolates were harvested. The positive result of HA test was continued to HI test. HI test was positive when there was an inhibition of agglutination. It was shown by the forming of erythrocytes sedimen like ring on the bottom of microplate’s wells. The result showed that from seventy samples, there was one sample infented by H1 of the pandemic H1N1-2009.

Keyword :

Swine Influenza, Pandemic H1NI-2009, Pig,

References :

Rotta P.A., E.P.Rocha, M.W. Harmon, V.S.Hinshaw, M.G.Sheerar, Y.Kawaoka, N.J. Cox and T.F.Smith,,(2001) Laboratory Characterization of a Swine Influenza Virus Isolated From A Fatal Case of Human Influenza. United States : J. of Clin. Microb. (27) 6: 1413-1416

Nidom, C.A,(2010) Pandemik Influenza H1N1 2009 Surabaya : Airlangga University Press

Archive Article

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Volume : 6 / No. : 1 / Pub. : 2013-02
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