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Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 1 / Published : 2010-02

TOC : 2, and page :5 - 10

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Original Article :

Isolation, identification and characterization of inhibin b protein of rattus norvegicus sertoli cell culture as male hormonal contraception candidate

Author :

  1. Aulanni'am*1
  2. Gatot Ciptadi*2
  3. Chanif Mahdi*3
  4. Muslim Akmal*4
  1. Fakultas MIPA Universitas Brawijaya, Malang
  2. Fakultas Peternakan Universitas Brawijaya, Malang
  3. Fakultas MIPA Universitas Brawijaya, Malang
  4. Mahasiswa Program Doktor Ilmu Kedokteran Program Pascasarjana Fakultas Kedokteran Universitas Brawijaya, Malang

Abstract :

The purpose of this study is to isolate, identify, and characterize the inhibin b protein of Rattus norvegicusSertoli cell culture. In this research, culture of Sertoli cell using by DMEM medium + FCS 15%. Confirmation ofinhibin b protein from Sertoli cell culture by dot blot technique used inhibin b polyclonal antibody. Identification andcharacterization of inhibin b protein from Sertoli cell culture by SDS PAGE. The result of this studies showed thatSertoli cell culture is the source of inhibin b protein with molecular weight of 175, 163, 153, 81, 78, 66, dan 29 kDa.

Keyword :

Inhibin beta, male hormonal contraception, ., ., .,


References :

  1. Amory, J.K. And W.J. Bremner, (2005). Oral testosteron in oil plus dutasteride: a pharmacokinetic study in men. . : J Clin Endocrinol Metab
  2. Mather, J.P., T.K. Woodruff and L.A. Krummen, (1992). Paracrine regulation of reproductive function by inhibin and activin. . : Proc Soc Ep Biol Med.


   


Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2010-02
  1. Apllication of frozen semen plus tyrosine kinase to increased conception rate of beef cattle
  2. Isolation, identification and characterization of inhibin b protein of rattus norvegicus sertoli cell culture as male hormonal contraception candidate
  3. Protective test immunogenic protein of outher membrane salmonella pullorum agains broiler
  4. Profile of combination ketamin xylazine and ketamin midazolam toward physiologycal change in male rabbit
  5. The feed's potential as the contamination source of aspergillus spp. which caused aspergillosis on fowl
  6. Metabolic product rumen fluid of sheep which given lactic acid bacteria suspension and yeast on king grass and rice straw
  7. Addition time interval effect of temu ireng (curcuma aeruginosa roxb.) extract to male mice (mus musculus) liver histopathological description
  8. Path analysis of mastitis on dairy cows at cooperative dairy farm in pasuruan, east java
  9. Viability and motility muscovy drake's (cairina moschata) spermatozoa in a diluter combination of skim milk, fructose and egg yolk
  10. Addition of growth differentiation factor-9 (gdf-9) at culture medium in vitro on chromosome profile of bovine's oocyte
  11. The potency of a black soybean milk and yellow soybean milk on the level of triglycerides in rat (rattus novergicus) fed with a high fat diet
  12. The effect of centrifugation on quality of motility, viability and acrosom cap of goat sperm
  13. Benzopyrene induce apoptosis of the lung cells in ratus norvegicus
  14. Partenogenetik development of mouse oocytes activation using ethanol and 6-dmap by in vitro
  15. Identification of pregnancy specific protein b (pspb) from placenta foetalis (cotyledon) freishian holstein cows