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Veterinaria Medika

ISSN 1979-1305

Vol. 10 / No. 1 / Published : 2017-02

TOC : 9, and page :61 - 70

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Original Article :

Pattern of antibiotics resistance on escherichia coli that isolated from raw milk in surabaya

Author :

  1. Hario Puntodewo Siswanto*1
  2. Mustofa Helmi Effendi*2
  3. Soetji Prawesthirini*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The main objective of this study was to describe the pattern of antibiotic resistancein Escherichia coli by using Antibiotics Sensitivity Test. Samples were taken from 40dairy cows in Surabaya. Each dairy cow milk samples taken as many as 10 ml of theudder. Samples were put in Brilliant Green Bile Broth (BGBB). Positive results in BGBBcharacterized by discolored BGBB, then the samples followed by wearing Eosin MethyleneBlue Agar (EMBA), and after it was suspected colonies Escherichia Coli (metallic green)confirmed by Indol test. Indol positive colonies, then tested with Antibiotics SensitivityTest. The results showed that 13 of 40 samples suspected of Escherichia coli in EMBAand also produce Indol test positive. Sensitivity Test Antibiotics the use of data resultingin as many as 13 samples of resistance to the antibiotic tetracycline 30 ug, 12 samplesshowed resistance to sulfonamides 25 mg and as many as 11 samples of Escherichia colibacteria resistant to Ampicillin 10 mg.

Keyword :

Escherichia coli, Antibiotics Sensitivity Test, Tetracycline, Sulfonamide, Ampicllin,

References :

  1. Aly, M. E. A., T. M. Essam and M. A. Amin, (2012). Antibiotic Resistance Profile of E. coli Strains Isolated from Clinical Specimens and Food Samples in Egypt.. 3: 176-182 : International Journal of Microbiological Research
  2. Hadiwiyoto, S, (1994). Teori dan Prosedur Pengujian Mutu Susu dan Hasil Olahannya.. 2: 160-169. : Liberty
  3. Harms, J.M., H. Bartels, F. Schlunzen, and A. Yonath., (2003). Antibiotics acting on the translational machinary.. 116: 1391- 1393. : J. Cell. Sci.


Archive Article

Cover Media Content

Volume : 10 / No. : 1 / Pub. : 2017-02
  1. Isolation and identification vibrio sp as vibriosis cause of agent at grouper fish (epinephelus fuscoguttatus) in banyuwangi regency
  2. Identification of species and stage of mite causes scabies by crusting severity on the rabbits
  3. Potency of curcumin on histopathological changes in the kidney of pregnant mice (mus musculus) exposed to cadmium
  4. Effect of vitamin c and e towards histopathological changes of duodenum in mice induced with borax
  5. The effects of crude protein bovine seminal plasma addition in skim milk and egg yolk diluent to motility, viability, dna fragmentation, and necrosis of ram spermatozoa after equilibration
  6. The effect of red fruit oil (pandanus conoideus lam.) on histopathology of follicle development in infertile mice modelling
  7. Prevalence and infection level of gastrointestinal worm nematode on long tailed macaque (macaca fascicularis) at taman wisata wendit malang regency
  8. Prevalence of distomatosis on various type of slaughtered cattle at penggaron semarang abattoir
  9. Pattern of antibiotics resistance on escherichia coli that isolated from raw milk in surabaya
  10. Effect of mangosteen (garcinia mangostana linn.) pericarp extracts on the diameter of mice (mus musculus) pre antral and antral follicles
  11. Semen quality, hsp 70 expression and dna damage of post thawing limousine bull spermatozoa after cooled at 5 °c
  12. The effects of propolis on histological liver in male mice (mus musculus)
  13. Effect of rosella flower (hibiscus sabdariffa l.) extract on liver histhopathologic of male mice (mus musculus) exposed plumbum acetate
  14. Effect of electrical field on the death of staphylococcus aures in milk
  15. The effect of mimosa pudica root extract to histopathological representation of rattus norvegicus liver injected with naja sputatrix venom
  16. Androgen dihydrotestosterone and its role in male reproductive system