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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

TOC : 6, and page :25 - 36

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Original Article :

Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c

Author :

  1. Susilo, Imam *1
  2. Gondo, Lisa *2
  3. Aji, Rahmad *3
  4. Bambang S.Z*4
  5. Khotib, Junaidi*5
  1. Departemen Patologi Anatomi, Fakultas Kedokteran Universitas Airlangga
  2. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga
  3. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga
  4. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga
  5. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga

Abstract :

Chronic pain, such as inflammatory pain, is difficult to manage. N-Methyl-D-Aspartate (NMDA) receptor is a key molecule involved in the pain pathway and sensitization. The present study was design to investigate the efficacyof a novel potent non-competitive NMDA receptor antagonist, MK-801, in relieving inflammatory pain in mice. A model of inflammatory pain state was induced by intraplantar injection of Complete Freund's Adjuvant (CFA). NMDA receptor antagonist MK-801 was administered intrathecally once a day for 7 consecutive days at 0.01, 0.10, 1.00, 10.00, or 20.00 nmol doses a week after CFA injection. Thermal hyperalgesia was measured on days 0, 1, 3, 5, 7,8, 10, 12, 14, and 21 after CFA injection by warm plate. Paw thickness at the ipsilateral site was also measured on days 0, 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, and 21 after CFA injection. Histology of the spinal cord tissue was examined by lightmicroscope following haematoxylline-eosin staining. The results showed that MK-801 given at 0.01-20.00 nmoldosage significantly increased mice's latency on thermal stimulation compared with placebo (p<0.001). Pawthickness was also significantly decreased to compare with placebo after intrathecal injection of MK-801 at 0.01, 1.00and 10.00 nmol dosage (p<0,001; p=0,005; p=0,015 respectively). Whereas MK-801 administration at 0.01, 1.00, and20.00 nmol could decrease the inflamatory cells infiltration and recover the dorsal horn histology compare with placebo. Taken together, these results show that NMDA receptor antagonist, MK-801, is effective in relieving the inflammatory pain.

Keyword :

Chronic Pain, Inflammatory Pain, MK-801, NMDA receptor, .,


References :

  1. Lipton, S., A., (2004). Failures and Successes of NMDA Receptor Antagonists: Molecular Basis for the Use of Open- Channel Blockers like Memantine in the Treatment of Acute and Chronic Neurologic Insults. United States : J. Am. Soc. Exp. Neuro Therapeutics, 1 :101-110.
  2. Kocaeli, H., E. Korfali, H. Öztürk, N. Kahveci dan S. Yılmazlar, (2005). MK-801 Improves Neurological and Histological Outcomes after Spinal Cord Ischemia Induced by Transient Aortic Cross-Clipping in Rats. United States : Surgical Neurology, 64 (2) : S22-S26.
  3. Alilain, W. J. dan H. G. Goshgarian, (2007). MK-801 Upregulates NR2A Protein Levels and Induces Functional Recovery of the Ipsilateral Hemidiaphragm Following Acute C2 Hemisection in Adult Rats. United States : The Journal of Spinal Cord Medicine. 30 (4) : 346-354


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
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  5. Avian influenza h5n1 vaccine candidate for chicken from east java isolate virus
  6. Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c
  7. Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture
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