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Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

TOC : 1, and page :1 - 4

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Original Article :

Production and specificity testing of monoclonal antibodies to bovine zonna pellucida 3 deglycosylated (mab-bzp3dg) for woman immunocontraceptive vaccine

Author :

  1. Sutiman B. Sumitro *1
  2. Aulanni'am *2
  3. Ciptadi. G *3
  4. Soewarto. S*4
  1. Biomolecular Laboratory, Biology Department
  2. Biochemistry Laboratory, Chemistry Department , Faculty of Mathematic and Natural Sciences
  3. Central Labolatory Of Life Sciences
  4. Departmen of obstetric and ginecology, RSSA, Brawijaya University

Abstract :

Immunocontraception has received increased interest in last decades as a potential way of controlling the rapidgrowth of Indonesian population. Development of vaccine based on zona pellucida antigens represents a promising approach to potential contraception. Recent advances in monoclonal antibody technology are enabling the development of new methods for producing specifif antibody for immunocontraception. Seven research stages wereconducted to construct monoclonal antibodies to Bovine Zonna Pellucida 3 Deglycosylated (MAb-bZP3dG). Stage1, collection Bovine Zonna Pellucida 3 Deglycosylated (bZP3dG), stage II, Immunisized mouse Balb/c by Bovine Zonna Pellucida 3 Deglycosylated (bZP3dG), stage III, Collecting spleen cells from a mouse that has been immunized with the desired antigen, stage IV  Fussion spleen cells with myeloma cells, stage V, Hybridoma screening, stage VI, single cell cloning and Monoclonal antibody production and characterization. The Resultshowed that MAb-bZP3dG were produced both hybridoma cell and ascites fluid positively and more specific reacted with antigen Bovine Zonna Pellucida 3 Deglycosylated (bZP3dG) by immunoblotting technique. MAbbZP3dG should prove useful for candidate immunocontraceptive vaccine.

Keyword :

Bovine Zonna Pellucida 3 Deglycosylated (bZP3dG), Immunocontraception, Monoclonal antibody, ., .,


References :

  1. Davis W.C, (1995). Methods in Molecular Biology, Vol. 45. Monoclonal antibody protocols.. Totowa, NJ, USA, 264 : Humana Press Inc.:
  2. Goldsby R.A, (2000). Immunology. California : W. H. Freeman and Company
  3. Kuby J. , (2004). The Anatomy of The Immune System. United Kingdom : Micro.msb.le.ac.uk
  4. Tulsiani D. R, (2000). Structural analysis of asparagine-linked glycan units of ZP2 and ZP3 glyci proteins from mouse zona pellucida. United States : Arch Biochem Biophys Oct 15 ; 382(2): 275-83
  5. Bog-Hansen T.C. , (1995). Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose.. United States : Methods in Molecular Biology 45:177-81


   


Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
  1. Production and specificity testing of monoclonal antibodies to bovine zonna pellucida 3 deglycosylated (mab-bzp3dg) for woman immunocontraceptive vaccine
  2. Potency of crude spirulina on protein efficiency ratio in laying hen
  3. H-y antisera preparation and x chromosomal receptor tracer as sex determination prototype
  4. Erythrocyte's form changes in dog's blood smear before and after the storage using citrate phosphate dextrose
  5. Avian influenza h5n1 vaccine candidate for chicken from east java isolate virus
  6. Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c
  7. Exploration cellulolytic of bacterium of rumen liquid beef cattle as inoculum of waste agriculture
  8. Respon hmg toward ovarium development in goat
  9. Identification of pregnancy associated glycoprotein (pag) from milk of pregnant dairy cattle
  10. The role of soil as a helminths transmitter around the habitas of babirusa
  11. Potency of brown seaweed (sargassum duplicatum bory) ethanol and ethyl acetic fraction to malondialdehyde concentration decreasing and histological retriveal of ibd (inflammatory bowel disease) rat small intestinal jejunum
  12. Blood glucose and total blood protein profile in sheep provided with lactic acid bacteria and yeast on king grass and rice straw
  13. Effect of pegagan (centella asiatica) extract in ovariectomized wistar-strain rattus norvegicus on epithelial proliferation of vaginal wall
  14. Excessive dose of vitamine a on skeletal development in the mice embryos