UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 2 / Published : 2013-07

Order : 10, and page :127 - 132

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Original Article :

Profil protein hemaglutinin (ha) berdasarkan berat molekul virus avian influenza isolat lokal

Author :

  1. Adhitya, Helmi*1
  2. Ernawati, Rahayu *2
  3. Plumeriastuti, Hany*3
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this study was to investigate protein molecular weight (mw) of Hemagglutinin of Avian Influenza virus. The influenza virus subtype H5N1 used in this study was propagated in embryonated chicken eggs and incubated at 370C temperature for five days. The first step, the samples were identified by SDS-PAGE (Sodium Dodecyl Sulphate–Poly Acrylamide Gel Electrophoresis ). The second step, thesamples were analyzed by Western Blot which was detected about protein reactivity toward AI polyclonal antibody. The result of the research showed that molecular weight of Hemagglutinin (HA) of the samples were  76,91 kDa. That protein were antigenic protein because it showed reactivity when were reacted with AI polyclonal antibody.

Keyword :

Avian Influenza, Hemagglutinin, SDS-Page, Western blot,


References :

Matrosovich, M.N., T.Y., Matrosovich, T. Gray, N.A. Robert, and H.D. Klenk.,(2004) Human and Avian Influenza Viruses Target Different Cell Types in Cultures of Human Airway Ephithelium. . : PNAS. 101 (13) : 4620 – 4624.

Suwarno.,Adi Prijo Rahardjo, Fauziah, Eko Agus.,(2006) Karakterisasi Virus Avian Influenza dengan Uji Serologik dan Reserve Transcriptase-Polymerase Chain Reaction. Surabaya : Media Kedokteran 22 (2) : 74-78.





Archive Article

Cover Media Content

Volume : 6 / No. : 2 / Pub. : 2013-07
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  9. Comparison Of Hemocytometer Thoma And Spectrophotometer Method On Sperm Concentration Examination Of Merino Sheep Semen
  10. Profil Protein Hemaglutinin (ha) Berdasarkan Berat Molekul Virus Avian Influenza Isolat Lokal
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