Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 2 / Published : 2013-07

Order : 10, and page :127 - 132

Related with : Scholar   Yahoo!   Bing

Original Article :

Profil protein hemaglutinin (ha) berdasarkan berat molekul virus avian influenza isolat lokal

Author :

  1. Adhitya, Helmi*1
  2. Ernawati, Rahayu *2
  3. Plumeriastuti, Hany*3
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this study was to investigate protein molecular weight (mw) of Hemagglutinin of Avian Influenza virus. The influenza virus subtype H5N1 used in this study was propagated in embryonated chicken eggs and incubated at 370C temperature for five days. The first step, the samples were identified by SDS-PAGE (Sodium Dodecyl Sulphate–Poly Acrylamide Gel Electrophoresis ). The second step, thesamples were analyzed by Western Blot which was detected about protein reactivity toward AI polyclonal antibody. The result of the research showed that molecular weight of Hemagglutinin (HA) of the samples were  76,91 kDa. That protein were antigenic protein because it showed reactivity when were reacted with AI polyclonal antibody.

Keyword :

Avian Influenza, Hemagglutinin, SDS-Page, Western blot,

References :

Matrosovich, M.N., T.Y., Matrosovich, T. Gray, N.A. Robert, and H.D. Klenk.,(2004) Human and Avian Influenza Viruses Target Different Cell Types in Cultures of Human Airway Ephithelium. . : PNAS. 101 (13) : 4620 – 4624.

Suwarno.,Adi Prijo Rahardjo, Fauziah, Eko Agus.,(2006) Karakterisasi Virus Avian Influenza dengan Uji Serologik dan Reserve Transcriptase-Polymerase Chain Reaction. Surabaya : Media Kedokteran 22 (2) : 74-78.

Archive Article

Cover Media Content

Volume : 6 / No. : 2 / Pub. : 2013-07
  1. The Influence Of Bacteriocin As A Preservation Of Beef To Extend Storage At 4°c-
  2. Influence Of Infusa Mangosteen Peel (gracinia Mangostana L) For Blood Glucose Levels Of Hyperglycemic Rats
  3. The Potential Of Red Roses Extract (rosa Damascena Mill) As An Antiseptic Stomatitis In Snakes (python Reticulatus) On The Total Number Leukocyte And Defferential Counting Of Leukocytes
  4. The Effect Of Green Bitter Melon Fruit Flesh (momordica Charantia L.) Extract On Mice (mus Musculus) Estrous Cycle Which Superovulated With Pmsg And Hcg
  5. The Antibiotica Susceptibility Of Escherichia Coli Isolated From Etawa Goat Milk
  6. Reproductive’s Efficiency Of Madura Cattle Artificial Insemination Result At Sumenep Regency In 2012
  7. Digestibility Value Of Proteinin Formula Feed For The Cats (felis Silvestris Catus)
  8. Effect Of Sambiloto (andrographis Paniculata) Extract Based On Histopathological Changes Of Gouramy (osphronemus Gouramy) Villi Intestine Infected Aeromonas Hydrophila
  9. Comparison Of Hemocytometer Thoma And Spectrophotometer Method On Sperm Concentration Examination Of Merino Sheep Semen
  10. Profil Protein Hemaglutinin (ha) Berdasarkan Berat Molekul Virus Avian Influenza Isolat Lokal
  11. The Effect Of Borax To Duodenal Histopathological Changes In Rats (rattus Norvegicus)
  12. Giving Probiotics To Increase Milk Production And Milk Proteins Of Dairy Cattle In Gresik Randu Padangan Village
  13. Differential Time Of Artificial Insemination On The Presentage Regnancy Of Fat Tailed Sheep
  14. Pengaruh Vaksinasi Dengue Multivalen Terhadap Ekspresi Toll-like Receptor Dan Sel T Cd4+ Pada Kelinci ( White New Zealand )
  15. Quality Test Of Frozen Semen Of Etawa Goat-breed’s In Three Different Kinds Of Extender With Water Incubator Examination