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Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 3 / Published : 2013-11

TOC : 4, and page :175 - 180

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Original Article :

Reactivity ib (infectious bronchitis) virus field isolate passaged on embryonated chicken 1 eggs against antibody post vaccination

Author :

  1. Retnani, T.D*1
  2. Suwarno*2
  3. Harijani, Nenny*3
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This study aims to determine the IB virus field isolates that react specifically to the results ofvaccination antibody (H120 and Mass + Conn), determine the effect of the passage of the field isolates of IBvirus and the interaction between the viral antigen IB field isolates and vaccine antibody results (H120 and Mass + Conn) as well as field isolates of IB virus antigen and passage. The allantoic fluid of virus IB isolatesI.4, I.8, and active vaccine virus from H120, cultured on Embryonated Chicken Eggs by passage three times.Antigen-antibody reactivity was read by ELISA method and is expressed in OD (Optical Density) values.This research used completely randomized design (CRD) 3 factors and the result was analyzed by using ANOVA (Analyzed of Variance), continued with Duncan’s Multiple Range Test method and processed byusing SPSS computer software program. The results of this research showed that isolates I.4 and I.8 havespecific reaction to antibodies (Mass + Conn and H120), but the reaction most intense in isolate I.8 (p<0.05),the highest passage on the third passage (p<0.05) and the interaction between antigens with antibodies isisolate I.8 with Mass+Conn (p<0.05). 

Keyword :

Infectious Bronchitis Virus, Field isolate, Passage, Antibodi, Optical Density,


References :

  1. Dharmayanti, NLP.I, W. Asmara, W.T. Artama, R. Indriani, dan Darminto, (2005). Hubungan Kekerabatan Virus Infectious Bronchitis Isolat Lapang Indonesia.. Bogor : Jurnal Bioteknologi Pertanian, 10(1) : 15-23.
  2. Gelb JrJ, Weisman Y, BS Ladman and Meir R., (2005). S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000).. United States : Avian Pathology 34:194-203.


   


Archive Article

Cover Media Content

Volume : 6 / No. : 3 / Pub. : 2013-11
  1. Effects of curcuma zedoariarosc.administration to dawley mice prior and after dmba initiation on the expression of cyp1a1 enzyme
  2. Effectiveness of crioprotectant during sperm freezing process using rapid freezing method toward the concentration of post thawing-frozen cow semen dna
  3. The effectiveness of garlic (allium sativum l.) extract in chicken broiler’s titer antibodies that vaccinated with live nd
  4. Reactivity ib (infectious bronchitis) virus field isolate passaged on embryonated chicken 1 eggs against antibody post vaccination
  5. Different animals species hairs as biological tool for the veterinary forensic assessement of three types of dogs, american pitbull terrier, german shepherd and doberman
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