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Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 3 / Published : 2008-11

TOC : 8, and page :113 - 118

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Original Article :

Reactivity of brucella abortus strain 19 outer membrane protein toward polyclonal antibody anti brucella abortus strain 19

Author :

  1. Ratih Ratnasari*1
  2. Desty Apritya*2
  3. Budi Utomo*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this research was to find out the reactivity of Brucella abortus S-19 outer membrane protein toward rabbit polyclonal antibody which have been injected with Brucella abortus S-19. In advanced it will be used as diagnostic kit that has sensitivity and highly specific for Brucellosis diagnosis.Brucella abortus S-19 was obtained from PUSVETMA bacteria stock. The suspention consist of Brucella abortus S-19 and the adjuvant was injected subcutaneous into rabbit in order to get polyclonal antibody anti Brucella abortus S-19. Booster has been done for six times.OMP was obtained from Brucella abortus S-19 by sonication technique, using ultrasonic homogenizer. The identification of immunogenic antigen protein fraction had done by western blot. Western blot technique used OMP antigen and polyclonal antibody (anti Brucella abortus S-19).The result of this research showed that OMP Brucella abortus S-19 have had five fractions. These protein had recognized antibody with molecul weight of : 55,3 kDa, 24,4 kDa, 21,9 kDa, 20,3 kDa and 14,7 kDa.

Keyword :

Outer Membran Protein, Brucella abortus S-19, Western blot, ., .,


References :

  1. Alton, G.G. Jones, L.M. Angus, R.D and Veyer J.M., (1988). Technique for the Brucellosis Laboratory. Institude Nasional de la Recherche. Paris : Agronomique
  2. Artama, W.T., (1991). Rekayasa Genetika. Yogyakarta : PAU – Bioteknologi Universitas Gajah Mada


   


Archive Article

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Volume : 1 / No. : 3 / Pub. : 2008-11
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  7. Toxoplasma gondii congenital dissemination number on infected pregnant mice by local isolate on difference gestation olds
  8. Reactivity of brucella abortus strain 19 outer membrane protein toward polyclonal antibody anti brucella abortus strain 19
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