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Veterinaria Medika

ISSN 1979-1305

Vol. 10 / No. 1 / Published : 2017-02

TOC : 11, and page :77 - 86

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Original Article :

Semen quality, hsp 70 expression and dna damage of post thawing limousine bull spermatozoa after cooled at 5 °c

Author :

  1. Anny Amaliya*1
  2. Suzanita Utama*2
  3. Hardijanto*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Good quality frozen semen is one of the factors that contribute to successfulartificial insemination. To get high fertility, spermatozoa should be added with extenderand cooled at 5°C for some time after collection. This study was aimed to know spermquality variables including motility, viability, abnormality, and plasma membraneintegrity as well as the expression of HSP 70 and the DNA damage of frozen semen aftercooling at 5°C for 4 hours and 22 hours one limousine bull. Semen was collected fromone Limosin bull twice a w week using artificial vagina. Following colection, semenwere evaluated macroscopicly and microscopically including viability, abnormality andplasma membrane integrity. Semen then cooled at 5°C for 4 and 22 hours. Before freezing,semen was cooled at 5°C for 4 and 22 hours. Motility, viability, abnormality, plasmamembrane integrity, expression of HSP 70 and DNA damage were evaluated before andafter freezing. In before freezing evaluation showed significant difference (p<0,05) onmotility beetween cooling at 5°C for 4 and 22 hours (55.6 ± 1.8 and 58,8 ± 2.3 %respectively), but there was no diference on viability, abnormality and plasma membraneintegrity, HSP 70 expression and DNA damage. Meanwhile, in post thawing no diferencewas found on motility, viability, abnormality, plasma membrane integrity, expression ofHSP 70 and DNA damage

Keyword :

cooling at 5°C, HSP 70, DNA damage,


References :

  1. Ariantie, O.S, T.L Yusuf, D. Sajuthi dan R.I Arifiantini. , (2013). Pengaruh Krioprotektan Gliserol dan Dimethilformamida dalam Pembekuan Semen Kambing Peranakan Etawah Menggunakan Pengencer Tris Modifikasi. . Vol. 18 No. 4: 239-250. : JITV
  2. Arifiantini, R.I dan B.Purwantara. , (2010). Motility and viability of Friesian Holstein semen in three different extender stored at 5°C. . 35:222-226. : J Indonesian Trop Anim Agric.
  3. Morrell, J.M and H. Rodriguez-Martinez. , (2009). Biomimetic techniques for improving sperm quality in animal breeding: a review. . 1 : 1-9. : The Open J Androl


   


Archive Article

Cover Media Content

Volume : 10 / No. : 1 / Pub. : 2017-02
  1. Isolation and identification vibrio sp as vibriosis cause of agent at grouper fish (epinephelus fuscoguttatus) in banyuwangi regency
  2. Identification of species and stage of mite causes scabies by crusting severity on the rabbits
  3. Potency of curcumin on histopathological changes in the kidney of pregnant mice (mus musculus) exposed to cadmium
  4. Effect of vitamin c and e towards histopathological changes of duodenum in mice induced with borax
  5. The effects of crude protein bovine seminal plasma addition in skim milk and egg yolk diluent to motility, viability, dna fragmentation, and necrosis of ram spermatozoa after equilibration
  6. The effect of red fruit oil (pandanus conoideus lam.) on histopathology of follicle development in infertile mice modelling
  7. Prevalence and infection level of gastrointestinal worm nematode on long tailed macaque (macaca fascicularis) at taman wisata wendit malang regency
  8. Prevalence of distomatosis on various type of slaughtered cattle at penggaron semarang abattoir
  9. Pattern of antibiotics resistance on escherichia coli that isolated from raw milk in surabaya
  10. Effect of mangosteen (garcinia mangostana linn.) pericarp extracts on the diameter of mice (mus musculus) pre antral and antral follicles
  11. Semen quality, hsp 70 expression and dna damage of post thawing limousine bull spermatozoa after cooled at 5 °c
  12. The effects of propolis on histological liver in male mice (mus musculus)
  13. Effect of rosella flower (hibiscus sabdariffa l.) extract on liver histhopathologic of male mice (mus musculus) exposed plumbum acetate
  14. Effect of electrical field on the death of staphylococcus aures in milk
  15. The effect of mimosa pudica root extract to histopathological representation of rattus norvegicus liver injected with naja sputatrix venom
  16. Androgen dihydrotestosterone and its role in male reproductive system