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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

TOC : 6, and page :134 - 139

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Original Article :

Spesifisity test with dot blotting of epidermal growth factor (egf) isolated from cumulus oocyte complex after in vitro maturation

Author :

  1. Widjiati*1
  2. Aulia Reza Pradipta*2
  3. Dady Soegianto Nazar*3
  4. A.T. Soelih Estoepangestie*4
  1. Dosen Fakultas Kedokteran Hewan
  2. Mahasiswa Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study was to know the character of epidermal growth factor (EGF) protein isolated from bovine oocyte. Bovine ovary was collected from slaughter house washed with NaCl. An aspiration technique was used to collect oocyte. Those oocytes were maturated by in vitro condition then the EGF protein was isolated after running in SDS PAGE. Polyclonal antibody was produced by sub cutaneous injected of crude protein oocytes containing EGF in rabbit, then followed by dot blotting examination. Booster was done twice after 3rd and 7th bleeding then continued until 10 times every week. The antibody anti EGF from rabbits sera was reacted with EGF antigen. The dot blot showed, the intensity of dot color increased significantly from 4th bleeding and reaches the highest concentration of antibody at 9th bleeding. It meant, bovine oocyte EGF protein could be characterized by dot blotting, of which the highest antibody titer detected at eleventh week after sub cutaneous injection of crude protein oocytes containing EGF.

Keyword :

Epidermal Growth Factor, In vitro Maturation, Dot Blotting,


References :

  1. Kobayashi, K., S.Yamashita and H. Hoshi, (1994). Influence of Epidermal Growth Factor And Transforming Growth Factor on In Vitro Maturation of Cumulus Cell-Enclosed Bovine Oocytes in a Defined Medium. . : J. Reprod. Fertil. 100: 439-446
  2. Lorenzo, P.L., I.K.M. Liu, J.C.Illera, R.A.Picazo, G.F.Carneiro, M.J. Illera, A.C. Conley, A.J.Ender, (2001). Influence of Epidermal Growth Factor on Mammalian Oocyte Maturation via Tyrosine-Kinase Pathway. . : J. Physiol. Biochem., 57 (1): 15-22
  3. Baratawidjaja, K.G, (2006). Imunologi Dasar. Jakarta : Penerbit Fakultas Kedokteran Universitas Indonesia


   


Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
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  5. Effect using a combination of mpa (medroxy progesterone acetate) and prostaglandin (pgf2α) injection on the percentage of estrous and pregnant on sheeps
  6. Spesifisity test with dot blotting of epidermal growth factor (egf) isolated from cumulus oocyte complex after in vitro maturation
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