UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 8, and page :289 - 300

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Original Article :

Suplementation of specific protein membrane spermatozoa in freezing medium to improvement efforts a succes cattle frozen semen quality post thawing

Author :

  1. Tatik Hernawati*1
  2. Sri Mulyati*2
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan

Abstract :

This research aims to improve the quality of post-thawing of frozen semen, with the addition of specific protein osteopontin spermatozoa membrane into a freezing medium. This research was conducted in the laboratory and in the field that includes the stages following methods frozen semen quality test supplemented osteopontin spermatozoa membranes isolated cow. The test includes: measuring the levels of osteopontin using the standard osteopontin ELISA, determination of the optimum dosage of the addition of osteopontin in freezing media on the quality of spermatozoa post thawing, which include : the percentage of motility, viability, plasma membrane intact and acrosome intact hood. Results showed that the addition of specific protein osteopontin membrane of the spermatozoa in the semen freezing diluter media there are significant differences (p < 0.05) compared with no addition of osteopontin this means that the addition of specific protein osteopontin membrane can increase the number of spermatozoa motility, viability, plasma membrane intact (MPU). The conclusion from this study is the addition of a specific protein osteopontin spermatozoa membrane can improve the quality of frozen semen and improvement of in vitro fertilization.

Keyword :

specific proteins, membrane spermatozoon, Diluter, Semen Quality,


References :

Anzar, M,, L. Hei, M. M. Buhr, T. G. Kroetsch and K. P. Pauls,(2002) Sperm Apoptosis in Fresh and Cryopreserved Bull Semen Detected by Flo Cytometry and Its Relationship with Fertility 66 :354-360 : Biol of Reprod

Caballero, J., G. Frenette and R. Sullivan,(2010) Post Testicular Sperm Maturational changes an Protosome 1 – 25 : Vet Medicine International

Martin, G., O. Sabido, P. Durand and R. Levy,(2004) Cryopreservation Induces an Apoptosis-Like Mechanism in Bull Sperm 71:28–37 : Biology of Reproduction





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
  7. Potency Of Apple Vinegar As Alternatif Therapy Against Wound Healing Time Of Staphylococcus Aureus Infection On Mice
  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

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