Veterinaria Medika
ISSN 1979-1305
Vol. 4 / No. 3 / Published : 2011-11
Order : 11, and page :213 - 220
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Original Article :
Supplement of acrosin to the post thawing sperm of half-breed of etawa goat (pe) towards capacitation and acrosome reaction of spermatozoa
Author :
- Budi Utomo*1
- Dosen Fakultas Kedokteran Hewan
Abstract :
The development of goats population in Indonesia has not reached a satisfactory condition, in East Java in the 2007 population of goats decreased for about 3,24%. The obstacle which is faced in the goats breeding field is involving reproduction, the problem includes failure sperm cell to penetrate zona pellucida on egg cell due to the of less potency enzyme acrosin and this is the first factor which hampers goats reproduction. The experiment was biological test for determining the potency of spermatozoa after thawing supplemented with acrosin at the dosages of 0; 3.0; 4.5 and 6.0 μg for 30 and 60 minutes respectively. This biological test including that capacity and acrosome reaction. The results were, in the first experiment supplement of acrosin dosage 4.5 μg increased capacity and spermatozoa goat acrosom reaction. The conclusion were supplement acrosin with dosage 4.5 μg increased quality of sperm
Keyword :
Acrosin, Quality Sperm, Biological Potency,
References :
Adel A. Zalata, H Ashraf, Ahmed and H Frank Comhaire,(2004) Relationship between acrosin activity of human spermatozoa and oxidative stress . : Asian J. Androl. Dec., 6, 2004 : 313-318
Arcelay, E. ;JG. Alvarez and BT Strorey,(2008) Asessment of Sperm Function for IVF . : J. Human Reprod. 3:89-95
Baldi, E; M Luconi, L Bonaccorsi, C Krausz and G. Forti,(2011) Human Sperm Activation During Capacitation And Acrosome Reaction: Role Of Calcium, Protein Phosphorylation And Lipid Remodelling Pathways . : Frontier In Bioscience 1. 189-205.
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Volume : 4 / No. : 3 / Pub. : 2011-11 |
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