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Veterinaria Medika

ISSN 1979-1305

Vol. 5 / No. 1 / Published : 2012-02

TOC : 7, and page :27 - 36

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Original Article :

Supplement of acrosin to the post thawing sperm of half-breed of etawa goat (pe) towards increasing potency of spermatozoa

Author :

  1. Utomo, Budi*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The development of goats population in Indonesia has not reached satisfactory condition, in East Java in the year 2007 the population of goat decreased for about 3,24 % (Anonimous, 2007). The governmenthas been trying to solve the problem concerning the decreas of goat population by using artificial insemination, however this effort has not been resulted in satisfactory condition. One of the main factor of the decreasing goat population is the reproduction problem, especially fertilization failure, in which  spermcell has not capable to fertilize the ovum. The failure of sperm cell penetration is caused by the reducing enzyme potency in the spermatozoa, especially acrosin enzyme that has function of the zona pelusida in theovum. In Indonesia, research about role and acrosin function in the fertilization, especially penetration on thezona pelusida in ovum of goats has not been reported yet. The general aim of the research was to determinethe role of acrosin towards the biological potency of spermatozoa to penetrate the zona pelusida. The specific aim of this research was to determine  effect of acrosin against the quality and the penetration of spermatozoa to the zona pelusida.  The  experiment was goat spermatozoa  supplemented with acrosin dosages 0; 3.0; 4.5 and 6.0 µg toward fertilization in vitro. The result of fertility test with acrosin supplemented 0; 3.0; 4.5; and6.0 µg the rate of cleavage of the oocyte was 40; 60; 60; and 0% respectively. In conclusion, dosage acrosin supplement 3.0 µg for 30 minutes was enough to induce cleavage.

Keyword :

Acrosin, Penetration of zona pelusida, Biological Potency,


References :

  1. Hermadi, H.A, (2009). Bioaktivitas Human Menopause Gonadotrophin (hMG) dan hMG Terdeglikolisasi Dari Urin Perempuan Pascamenopause Terhadap Terbentuknya Cleavage Embrio Sapi Secara In-Vitro. Surabaya : Disertasi. Program Pascasarjana Universitas Airlangga
  2. Duan, H., X. Zhang, Y. Li, M. Gao, L. Den and H. Feng, (2007). Study on the relationship of sperm acrosin activity with fertilization rate during in-vitro fertilization.. China : Chinese Journal of Andrology Pages 4246.
  3. Adel A. Zalata, H. Ashraf Ahmed and H. Frank Comhaire, (2004). Relationship between acrosin activity of human spermatozoa and oxidative stress. Shanghai : Asian J. Androl.: 313-318


   


Archive Article

Cover Media Content

Volume : 5 / No. : 1 / Pub. : 2012-02
  1. Feed convertion and feed consumption of feed formulation with different protein content
  2. The potential of complete feed fermentation use cellulolityc bacteria to increase daily gain of sheep
  3. The effectivity of psidium guajava linn leaf dipping to chicken egg yolk quality
  4. The role of location against gastrointestinal nematodosis on cattle at west madura
  5. The activity test of whole bacterium suspension of bacillus thuringiensis subspecies israelensis serotype h-14 at mosquito of culex fatigans pipiens and aedes aegypti
  6. The metabolite levels of broiler serum that suffer due to warming heat stress in the long term
  7. Supplement of acrosin to the post thawing sperm of half-breed of etawa goat (pe) towards increasing potency of spermatozoa
  8. Quality test of frozen semens of fat-tailed sheep in three different kinds of semen extenders
  9. The level of progesteron in blood sera cows using ria( radio immuno assay ) after progesteron transvaginal mpa (medroxy progesteron acetate)
  10. Model plastik t sponge mpa (medroxy progesteron acetate) soft release for oestrus synchronization in cows
  11. Eimeria necatrix pathogenicity attenuation through a serial passage of precocious lines towards small intestine histopathology in broiler
  12. The effect of ethanol extracted of curcuma aeruginosa roxb rizhoma toward degree”s of kidney damage chicken layer which it is infected by ascridia galli
  13. Fluctuation of interleukin-4 ( il-4) in serum and reproductive organ at toxoplasma gondii infected pregnant mouse
  14. Expression difference of Αvβ3 in the endometrium after the administration clomiphene citrate, combination of clomiphene citrate and metformin, or metformin
  15. A comparison of the effect melatonin and forbetes on blood glucose level in rat (rattus norvegicus) experimentally diabetic mellitus type 1