UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 4, and page :263 - 270

Related with : Scholar   Yahoo!   Bing

Original Article :

Supplementation of insulin transferrin selenium on in vitro maturation cumulus oocyte complex (coc) against to mitogen activated protein kinase (mapk) expression and expanded cumulus mass

Author :

  1. Galuh Chandra Agustina*1
  2. Widjiati*2
  3. Pudji Srianto*3
  4. Hani Plumeriastuti*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the increase of expanded cumulus and MAPK expression as the promoting re-meiosis in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.5 0C in incubator CO2 5% for 24 hours. The result supplementation ITS P0 (0 μg/ml) was significantly different (p<0,05) with P1(10 μg/ml), P2 (15 μg/ml) and P3(20 μg/ml) in increased the expended cumulus mass and MAPK expression in Cumulus Oocyte Complex (COC). Expanded cumulus mass was not significantly different (p>0,05), while MAPK expression was significantly different (p<0,05) at P2 (15 μg/ml) and P3(20 μg/ml). The conclusion supplementation ITS on maturation medium able to increase expanded cumulus mass as well as the MAPK expression in oocytes maturation.

Keyword :

Oocytes, IVM, Insulin Transferrin Selenium, expanded cumulus mass, MAPK,


References :

Adifa, NS., P. Astuti, dan T. Widayati,(2010) Pengaruh Penambahan Chorionic Gonadotrophin Pada Mediu Maturasi Terhadap Kemampuan maturasi, Fertilisasi, Dan Perkembangan Embrio Secara In Vitro Kambing Peranakan Ettawa 34(1): 8-15 : Buletin Peternakan

Bowles, CM. and AW. Lishman,(1998) Attempts to Improve the Yield of Bovine Blastocysts by Incorporating Insulin, Selenium, and Transferrin in the In Vitro System 28 (1) : S Afr. J Anim Sci

Djuwita, I., V. Riyacumala, K. Mohamad, WE. Prasetyaningtijas, dan Nurhidayat,(2012) Pertumbuhan dan Sekresi Protein Hasil Kultur Primer Sel-sel Serebrum Anak Tikus 13 (2):125-135. : Jurnal Veteriner





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
  7. Potency Of Apple Vinegar As Alternatif Therapy Against Wound Healing Time Of Staphylococcus Aureus Infection On Mice
  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

Information VETMED