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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 3 / Published : 2014-01

TOC : 1, and page :200 - 207

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Original Article :

The accuracy of production equine chorionic gonadotropin (ecg) frozen dry from local horse for increasing the time onset of oestrus in madura cattle

Author :

  1. Herry Agoes Hermadi*1
  2. Arief Budhyantoro*2
  3. Tokok Ardianto*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Fakultas Sains dan Teknologi Universitas Airlangga
  3. Fakultas Sains dan Teknologi Universitas Airlangga

Abstract :

The aim of this study was to produce eCG from the local pregnant mares sera. This study identified eCG by conformation of the glycoprotein characteristic. The pregnant mares sera were collected from 10 horses. The protein filtrated by using sephadex G-100. The result of product eCG it can be frozen dry eCG. The Continued of this study was to evaluate biopotency of eCG from local Indonesian horse and eCG from paten product (PG 600 Intervet Holland) to onset of estrus time, and pregnancy rate of Madura cattle in Sembilangan Village Bangkalan district East Java. This study used 24 female Madura cattle divided by 4 treatment group. All of the cattles were being syncronized of their estrus cycle. After that control group (K) was injected by 600 IU PG 600 Intervet holland, P1, P2 and P3 were injected by eCG local horse product with dosage 600 IU, 300 IU and 150 IU respectively. After the injection of eCG the onset of estrus time were being evaluate. And than using two dimention USG follicle number were examined. The result of this study showed that there were no significance different of onset estrus time between control, P1, P2 and P3 group.

Keyword :

Pregnant mares sera, eCG, Frozen dry eCG, The time of oestrus,


References :

  1. Lunenfeld B., (2004). Historical perspectives in gonadotrophin therapy. . : Hum Reprod Update,10: 453-67
  2. Schally A.V., A. Arimura, A.J. Kastin, H. Matsuo, Y. Baba, T.W. Redding, R.M. Nair, L. Debeljuk and , (1971). Gonadotropin-releasing hormone: one polypeptide regulates secretion of luteinizing and follicle-stimulating hormones. . : Science. 173: 1036-1038
  3. Widjaja N.M.R, (2006). Pemisahan Equine Chorionic Gonadotropin (eCG) dari serum kuda bunting serta uji Potensi Biologisnya. Surabaya : Disertasi Program Pasca Sarjana Universitas Airlangga


   


Archive Article

Cover Media Content

Volume : 7 / No. : 3 / Pub. : 2014-11
  1. The accuracy of production equine chorionic gonadotropin (ecg) frozen dry from local horse for increasing the time onset of oestrus in madura cattle
  2. Effect of infusum kopi leaves (coffea robusta lindl.) on blood glucose level on the mice in alloxan induced
  3. Comparison of sperm quality after separation x and y chromosome between column albumin method and electric separating sperm (ess) method on ekor gemuk sheep
  4. Potential of vitamin e on spermatogenic cell number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  5. The comparison of maturation between bovine oocyte vitrification before and after in vitro maturation
  6. Potency of pediococcus pentosaceus protease on meat tenderness and histological
  7. Effect of vitamin e on decreased of the leydig cell necrosis number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  8. The immunomodulator potency of combination of andrographis paniculata and echinacea purpurea on total and differential leucocytes count in mice with heat stress
  9. The effect of various egg duck concentration in skim milk as semen diluters of fat-tailed sheep for motility, viability and intact plasma membrane spermatozoa before freezing
  10. Antibacterial effect of mango’s leaves (mangifera indica) extract against staphylococcus aureus and escherichia coli
  11. Effectiveness of honey and the juice of noni (morinda citrifolia linn) as well as mixtures of the number of bacteria on chicken carcasses
  12. The potential of soursop (annona muricata linn.) leaves extract as immunostimulantin rabbit pbmc
  13. Crude protein and crude fiber in rice bran fermented by acidothermus cellulolyticus or aspergillus terreus wpl 109 or wpl 209
  14. Level of contamination of salmonella sp in white prawns (litopenaeus vannamei) sold at some wet and modern market in surabaya
  15. Potensi alkaloid achyranthes aspera linn terhadap ekspresi protein bax sel kanker payudara pada mencit (mus musculus) yang diinduksi benzopyrene