Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 6 / No. 1 / Published : 2013-02

Order : 3, and page :97 - 14

Related with : Scholar   Yahoo!   Bing

Original Article :

The colour differences between escherichia coli and pseudomonas aeruginosa colonies in artificial medium that was made from beef extract, mung beans juice, citrate and bromthymol blue

Author :

  1. Muhaimin, A.A*1
  2. Siswanto, H.P*2
  3. Tyasningsih, Wiwiek*3
  4. Sarudji, Suryanie*4
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This research was aimed to investigate the colour differences between Escherichia coli and Pseudomonas aeruginosa colonies in artificial medium that was made from beef extract, mung beans juice, citrate and bromthymol blue. The medium was made by mixing 1.6% agar base, 0.8% beefextract, 20% mung beans juice, 0.2% sodium citrate, 2% lactose, and 8% of bromthymol blue solution0.1% into 1000 ml aquadest. The isolate of Escherichia coli and Pseudomonas aeruginosa wereinoculated onto the media with five repetitions. As control, Escherichia coli and Pseudomonasa eruginosa were inoculated onto the media that were made from beef extract and mung beans juice, without citrate and bromthymol blue. The whole media were incubated at 37o C for 24 hours. The result showed that the colour of Escherichia coli colonies can be distinguished with Pseudomonas aeruginosa’s. Escherichia coli colonies appeared colorless, wet, shiny and the surrounding medium remained yellow. Pseudomonas aeruginosa colonies appeared bluish-white, with thick and mucoid form and the surrounding medium changed its colour to green. Based on the result, the artificial medium that was made from beef extract, mung beans juice, citrate and bromthymol blue can be used in Microbiology Laboratories as a differential medium to distinguish bacteria whether they utilize citrate as energy and carbon source or not.

Keyword :

Artificial medium, Beef extract, Citrate,

References :

Drider, D., S. Bekal, and H. Prevost.,(2004) Genetic Organization and Expression of Citrate Permease in Lactic Acid Bacteria. . : Genet. Mol. Res. 3(2): 271-281.

Suryanie.,(2001) Pembuatan Media Pertumbuhan Kuman Streptococcus pyogenes dan Salmonella pullorum Dari Ekstrak Daging Sapi Yang Ditambah Sari Kacang Hijau. Surabaya : Media Kedokteran Hewan 17(1): 18-22. Fakultas Kedokteran Hewan Universitas Airlangga.

Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2013-02
  1. The Level Of Capric Acid Milk Cow On Consuming Complete Feed
  2. Injection Effect Of Mice (mus Musculus) Testes And Spleen Homogenat Derived Hy-antigen On Pup’s Sex Ratio
  3. The Colour Differences Between Escherichia Coli And Pseudomonas Aeruginosa Colonies In Artificial Medium That Was Made From Beef Extract, Mung Beans Juice, Citrate And Bromthymol Blue
  4. Effects Of Beef Packaging And Storage Temperature On The Early Beef Spoilage
  5. Comparasion Of Chicken Humoral Immune Response Vaccinated With Active Ibd Lv-13ua And Lv-14ua Under Value Optical Density Indirect Elisa
  6. Effectivity Of Gynura Procumbens Extract To Inhibit Vascular Endothelial Growth Factor (vegf) Expression On New Blood Vessels Of Chorioallantois Membran (cam) Chicken Embryonal
  7. Motility And Viability Thick Tail Ram’s Spermatozoa On Various Silver Ion (ag ) Concentration In Egg Yolk Citrate Coconut Water Assortment Extender
  8. The Isolation Of Escherichia Coli From The Meat Some Traditional Markets In The South Surabaya
  9. Conception Rate, Services Per Conception, Dan Calving Rate Setelah Ib Pada Sapi Potong Di Kabupaten Tulungagung Periode Januari – Desember 2010
  10. Motility And Life Percentage Of Friesian Holstein Bull’s Post Thawing Sperms In Egg Yolk Skim, Egg Yolk Tris And Andromed® Extenders
  11. Non Beta Lactam Antibiotics Resistance In Coagulase Positive Staphylococcus Isolated From Canine Pyoderma
  12. Study The Couples Of Bali Starlings (leucopsar Rothschildi) On Breeding Cages At Surabaya Zoo
  13. The Quality Of Etawa Goat-breed’s Frozen Semen In Various Types Of Diluent
  14. Identification Pandemic H1n1-2009 Virus On Pigs In Slaughterhouse Pegirian Surabaya By Using Hi Test
  15. Potential Of Concentrate Feed And Lactation Periods On Milk Protein And Feed Conversion On Dairy Cattle Friesian Holstein Crossbred