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Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 3 / Published : 2008-11

TOC : 3, and page :87 - 92

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Original Article :

The comparison between protein a expression of staphylococcus aureus and coagulase negative staphylococci (cns) from bovine mastitis milk

Author :

  1. Budiarto*1
  2. Mustofa Helmi Effendi*2
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The experiment was done to show the comparison between expression of encoding gene for bacterial protein A of Staphylococcus aureus and Coagulase Negative Staphylococci (CNS). The first step of test was to prepare pure culture of Staphylococcus aureus and and Coagulase Negative Staphylococci. Milk samples were collected from mastitic cases at the afternoon milking time. Preparation of pure culture were confirmed by MS agar, hemolytic activity, catalase and coagulase test. The result showed that characterization of molecular weight of protein A from Staphylococcus aureus was 55 kDa and there was no expression of encoding gene for bacterial protein A from Coagulase Negative Staphylococci.

Keyword :

Protein A, Staphylococcus aureus, Coagulase Negative Staphylococci, molecular weight 55 kDa, .,


References :

  1. Akineden, O., C. Annemuller, A.A. Hassan, C. Laemler, W..Wolter and M. Zschok, (2001). Toxin Genes and Other Characeristics of Staphylococcus aureus Isolates from Milk Cows with Mastitis. . : Clinical and Diagnostic Laboratory Immunology
  2. Hurley , W.L and D.E. Morin, (2003). Mastitis Lesson A. Illinois, USA : University of Illinois


   


Archive Article

Cover Media Content

Volume : 1 / No. : 3 / Pub. : 2008-11
  1. Effect of justicia gendarussa burm.f. leaves water fraction on male rabbit liver and renal fuction(sub acute toxicity test of justicia gendarussa burm.f. leaves water fraction as male contraceptive agent)
  2. Isolation and identification of staphylococcus aureus isolates from milk of bovine mastitis
  3. The comparison between protein a expression of staphylococcus aureus and coagulase negative staphylococci (cns) from bovine mastitis milk
  4. Histopatology and proliferation activity of gland mammae after gift curcuma zedoaria extract which dmba (dimethylbenz(a)antrasen) initiation on sprague dawley rat
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  6. The histopathology description of white rat (rattus norvegicus) intestine that was given juice of noni (morinda citrifolia) and high fatty diet
  7. Toxoplasma gondii congenital dissemination number on infected pregnant mice by local isolate on difference gestation olds
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  10. The increasing of rice straw quality by addtion cellulolityc bacteria produce microbe of rumen
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  12. Succesfull of artifical insemination using frozen semen carried by ice
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