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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 3 / Published : 2014-11

TOC : 5, and page :232 - 239

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Original Article :

The comparison of maturation between bovine oocyte vitrification before and after in vitro maturation

Author :

  1. Zakiyatul Faizah*1
  2. Aucky Hinting*2
  3. Ninik Darsini*3
  4. Widjiati*4
  1. Fakultas Kedokteran Universitas Airlangga
  2. Fakultas Kedokteran Universitas Airlangga
  3. Fakultas Kedokteran Universitas Airlangga
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The incidence of cancer in whole world is increase and shifted to younger age (productive), no exception incidence of ovarian cancer. Women who are diagnosed with cancer at the productive age do not only need to get attention on survival but also on the possibility of the loss of fertility.Vitrification method has become a growing and promising technology in assisted reproductive technology. Freezing embryos are more common, freezing oocytes are longer growing because the physical characteristics of oocyte. Vitrified oocytes can be performed on mature and imature oocytes to get for more back up, therefore it is necessary to develop systems for freezing and oocyte maturation This study aims to compare the number of matur oocyte between bovine oocyte vitrification pre and post in vitro maturation. Maturation is operated in medium TC 100 μl covered with mineral oil in a petri dish with diameters 36 mm. Vitrification begins with washing oocyte in PBS of 20% serum for 1-2 minutes, followed by PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred in 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. This study conclude that no difference on matur oocyte number between bovine oocyte vitrification pre and post in vitro maturation.

Keyword :

Vitrification, In vitro maturation, Oocyte, Bovine,

References :

  1. Adona, P.R., P.R.L.Pires, M.D. Quetglas, K.R.L.Schwarz, and C.L.V. Leal, (2008). Nuclear maturation kinetics and in vitro embryo development of cattle oocytes prematured with butyrolactone I combined or not combined with roscovitine. . : Animal Reproduction Science. 104:389–397
  2. Diez, Carmen, Paloma Duque, Enrique Go´mez, Carlos O. Hidalgo, Carolina Tamargo, Aida Rodrı´guez,, (2005). Bovine oocyte vitrification before or after meiotic arrest. . : Theriogenology. 64: 317-333
  3. Gardner, D.K., Btros R.M.B Rizk, and Tommaso Falcone, (2011). Human assisted reproductive technology: future trends in laboratory and clinical practice. UK : Cambridge university press. 314


Archive Article

Cover Media Content

Volume : 7 / No. : 3 / Pub. : 2014-11
  1. The accuracy of production equine chorionic gonadotropin (ecg) frozen dry from local horse for increasing the time onset of oestrus in madura cattle
  2. Effect of infusum kopi leaves (coffea robusta lindl.) on blood glucose level on the mice in alloxan induced
  3. Comparison of sperm quality after separation x and y chromosome between column albumin method and electric separating sperm (ess) method on ekor gemuk sheep
  4. Potential of vitamin e on spermatogenic cell number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  5. The comparison of maturation between bovine oocyte vitrification before and after in vitro maturation
  6. Potency of pediococcus pentosaceus protease on meat tenderness and histological
  7. Effect of vitamin e on decreased of the leydig cell necrosis number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  8. The immunomodulator potency of combination of andrographis paniculata and echinacea purpurea on total and differential leucocytes count in mice with heat stress
  9. The effect of various egg duck concentration in skim milk as semen diluters of fat-tailed sheep for motility, viability and intact plasma membrane spermatozoa before freezing
  10. Antibacterial effect of mango’s leaves (mangifera indica) extract against staphylococcus aureus and escherichia coli
  11. Effectiveness of honey and the juice of noni (morinda citrifolia linn) as well as mixtures of the number of bacteria on chicken carcasses
  12. The potential of soursop (annona muricata linn.) leaves extract as immunostimulantin rabbit pbmc
  13. Crude protein and crude fiber in rice bran fermented by acidothermus cellulolyticus or aspergillus terreus wpl 109 or wpl 209
  14. Level of contamination of salmonella sp in white prawns (litopenaeus vannamei) sold at some wet and modern market in surabaya
  15. Potensi alkaloid achyranthes aspera linn terhadap ekspresi protein bax sel kanker payudara pada mencit (mus musculus) yang diinduksi benzopyrene