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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 1 / Published : 2014-02

TOC : 4, and page :23 - 30

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Original Article :

The comparison of morphology between bovine oocyte vitrification before and after in vitro maturation

Author :

  1. Zakiyatul Faizah*1
  2. Aucky Hinting*2
  3. Ninik Darsini*3
  4. Widjiati*4
  1. Mahasiswa Fakultas Kedokteran
  2. Mahasiswa Fakultas Kedokteran
  3. Mahasiswa Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

Vitrification method has become a growing and promising technology in assisted reproductive technology. Freezing embryos are more common, freezing oocytes are longer growing because the physical characteristics of oocyte. The spindle of oocytes can be maintained during the process of freezing and warming, although exposed to very cold temperatures and undergo twice transition phase.This study aims to compare the number of morphology between bovine oocyte vitrification pre and post in vitro maturation. Maturation is operated in medium TC 100 μl covered with mineral oil in a petri dish with diameters 36 mm. Vitrification begins with washing oocyte in PBS of 20% serum for 1-2 minutes, followed by PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred in 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose.This study conclude that no difference on normal and abnormal oocyte morphology between bovine oocyte vitrification pre and post in vitro maturation.

Keyword :

Vitrification, In vitro maturation, Morphology,


References :

  1. Alworth AE and D.F. Albertini, (1993). Meiotic maturation in cultured bovine oocytes is accompanied by remodeling of the cumulus cells cytoskeleton. . : Development Biology. 158:101-112
  2. Bogliolo L, F. Ariu, S. Fois , I. Rosati , M.T. Zedda , G. Leoni , S. Succu , S. Pau and S. Ledda, (2007). Morphological and Biochemical Analysis of Immature Ovineoocytes Vitrified With or Without Cumulus Cells. Theriogenology.. USA : Theriogenology. 68: 1138-1149
  3. Gardner, DK, B.R.M.B Rizk and T. Falcone, (2011). Human Assisted Reproductive Technology: Future Trends In Laboratory and Clinical Practice. UK : Cambridge University Press. 314


   


Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2014-02
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  2. Effect of infusum salam leaves (eugenia polyantha) on blood glucose level on the rat (rattus norwegicus) in alloxan induced
  3. Potential of phytase ezymes from rumen bacteria on sem analysis structural changes of rice bran as broiler feed
  4. The comparison of morphology between bovine oocyte vitrification before and after in vitro maturation
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