UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 2 / Published : 2010-07

Order : 1, and page :81 - 86

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Original Article :

The effect of caspase 3 (caspase excecutor) on apoptotic induction endometrial cancer cel by alkaloid achyranthes aspera linn in vitro

Author :

  1. Wurlina*1
  2. Meles, D.K*2
  3. Zakaria.S*3
  4. Sastrowardoyo,W*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Universitas Airlangga
  4. Fakultas Kedokteran Universitas Airlangga

Abstract :

The purpose these research to prove the alkaloid Acyranthes aspera Linn to influence on apoptotic induction in endometrial cancer. Prototipe of endometrial cancer use myeloma cell in vitro dan alkaloid of Achyranthes aspera Linn leaf was prepare by Pharmacope Indonesian methode. This research was proved on myeloma cell, devided into 3 groups: the first group (treatment groups) was given alkaloid fraction in concentration 1 ppm, 10 ppm, 100 ppm and 1000 ppm. The second group (negative control) was given RPMI and DMSO media only. The third groups (positivecontrol) given Colsemide in concentration 100 ppm. Each groups was represented with 4 hole in 24 microwell platehole.The result showed that the alkaloid fraction of Achyranthes aspera Linn in dose 100 ppm cause apoptotic myeloma cell 37,52 + 3,67% and higher than positive control 31,41 + 5,96%, caused necrotic myeloma cell 53,03 +2,42% lower than positive control 55,40 + 4,23% and the lethal concentration 50% on myeloma cell was 0,719 ppm. The optical density of caspase by Elisa test to begin at dose 10 ppm concentration of alkaloid Achyranthes aspera Linn (value of caspase > 2x COV).The conclution of these research the alkaloid Achyranthes aspera Linn cause of cell death of myeloma bynecrotic and apoptotic, and to increase of dose of alkaloid cause to increase the optical density of caspase.

Keyword :

Achyranthes aspera Linn, caspase, myeloma cell,


References :

Chaoi S.H.,S.Y.Lyu and W.B. Park,(2004) Mistietoe lectin Induce Apoptosis and Telomerase Inhibition In H u m a n A 2 5 3 c a n c e r C e l l s Through Dephosphorylation of Akt United States : Pharm Res

Chakraborty, A; A.Branther; T. Mukainaka; T . K o n o s h i m a ; H . T o k u d a a n d H.Nishino,(2002) Cancer hemopreventive Activity of Achyrantes aspera Leaves on Epstein Barr Virus Activation and Two-stage Mouse Skin Carcinogenesis Japan : Carcer Lett

Andrew W, D.Vicky,D.Hill, J.Keesey and S. Manzow,(2002) Cell Death. Apoptosis and N e c r o s i s P r o l i f e r a t i o n United States : B o e h r i g e r Mannheim





Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2010-07
  1. The Effect Of Caspase 3 (caspase Excecutor) On Apoptotic Induction Endometrial Cancer Cel By Alkaloid Achyranthes Aspera Linn In Vitro
  2. Detection Of Inflamentation On Duck Moulting Therapy By Anti-prolactine Injection
  3. The Effects Of Eucheuma Spinosum Extracts On The Sod (superoxide Dismutase) Activities On Mice (rattus Novergicus) Induced Multiple-low Dose Streptozotocin
  4. Ethanol Extract Of Brown Secured (sargassum Duplicatum Bory): Phytopharmaco Exploration For Lung Cancer Therapy
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  14. Influence Of Melatonin To Histopathology Pancreas In Rat ( Rattus Norvegicus ) Experimentally Diabetes Mellitus Type I

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