Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 2 / No. 2 / Published : 2009-07

Order : 5, and page :105 - 108

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Original Article :

The effect of cryoprotectan combination dimethyl sulfoxyde (dmso) and glycerol at frozen semen extender of cattle for spermatozoa quality pasca thawing

Author :

  1. Trilas Sardjito*1
  2. Ristina Windawati*2
  3. Adrianan Monica Sahidu*3
  4. Sri Pantja Madyawati*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this research was to know that the combination of cryoprotectan Dimethyl Sulfoxide (DMSO) and glycerol at frozen semen extender of bull could increased the motility percentage of spermatozoa and the living of spermatozoa pasca thawing. Bull semen of Limousine age two year was collected used artificial vagina, than made in one treatment and one control. P0 as control (semen, glucose, glycerol 8%, milk skim diluter-egg yolk) and P1 as treatment (semen, glucose, DMSO 1%, glycerol 6%, milk skim diluter-egg yolk), the continued with frozen procces and examination of Pasca Thawing Motility (PTM). The data were analyzed by Paired Samples T Test. The result showed that higly significant difference (p<0,01) for control and treatment. Conclusively, combination of cryoprotectan DMSO 1% and glycerol 6% could increased the motility percentage of spermatozoa and the living of spermatozoa pasca thawing.

Keyword :

Combination DMSO 1% and glycerol 6%, spermatozoa, bull, ., .,

References :

Holt, W. V.,(2000) Fundamental Aspect of Sperm Cryobiology, The Importance of Species and Individual Differences . : Elsevier Science Inc.

Hafez, E.S.E and B. Hafez,(2000) Reproduction in Farm Animal Philadelphia. USA : 7 th Rd. Lippicott Williams & Wilkins

Direktorat Jendral Peternakan,(2000) Prosedur Tetap (PROTAP) Produksi dan Distribusi Semen Beku Jakarta : Departemen Pertanian Direktorat Jendral Peternakan

Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2009-07
  1. Protein Digestibility Of Laying Hens Added Suplementation Probiotic-chlorella
  2. Characterization Of Bacterial Surface Protein On Staphylococcus Aureus And Staphylococcus Epidermidis From Milk Cows With Mastitis
  3. The Sensitivity Of Coagulase Slide Test For Detection Of Staphylococcus Aureus On Bovine Mastitis Cases
  4. The Motility And Viability Of Entok (cairina Moschata) Spermatozoa In Diluter Of Skim Milk-fructosa
  5. The Effect Of Cryoprotectan Combination Dimethyl Sulfoxyde (dmso) And Glycerol At Frozen Semen Extender Of Cattle For Spermatozoa Quality Pasca Thawing
  6. Control Intra Vaginal Device Release (cidr) In Artificial Insemination Program Of Beef Catlle In Banyuwangi Region
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  9. Male Infertility: Sperm Dna Damage, Reactive Oxygen Species (ros) And The Key Role Of Protamine Molecule
  10. The Influence Of Lps ( Iipopolysaccharide) To Maltase Activity Isolated From Rattus Norvegicus Jejunum
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  14. The Effects Of Trehalose Level In Tris Based Diluter To Cross Breed Etawah Goat Spermatozoa Quality After Storage At Room Temperature
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  16. The Influence Of Bacteriocin Which Was Produced By Lactic Acid Bacteria Of Pediococcus Pentosaceus As A Teat Dip Substance Against Sub Clinical Mastitis