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Veterinaria Medika

ISSN 1979-1305

Vol. 10 / No. 1 / Published : 2017-02

TOC : 5, and page :31 - 40

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Original Article :

The effects of crude protein bovine seminal plasma addition in skim milk and egg yolk diluent to motility, viability, dna fragmentation, and necrosis of ram spermatozoa after equilibration

Author :

  1. Erry Tri Sheliana Adikara*1
  2. Suherni Susilowati*2
  3. Sri Pantja Madyawati*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to determine the effects of crude protein bovine seminalplasma addition in skim milik and egg yolk diluent in increased motility and viability aswell as decreased of DNA fragementation and necrosis of ram spermatozoa (Domba EkorGemuk). This study was used Complete Random Design with six replications and threetreatments. Control treatment (P0) is consist (ram semen + diluent); treatment I consistof (ram semen + diluent) crude protein bovine seminal plasma in the 1:1 proportion;treatment II consist of (ram semen + diluent) crude protein bovine seminal plasma in the1:2 proportion, then stored at 5°C. All treatments examined after 1 hour after equilibration.The results showed that addition of crude protein bovine seminal plasma (1:1 proportion)in skim milk and egg yolk diluent gave siginificant effects on motility, viability, DNAfragmentation, and necrosis of ram spermatozoa, whereas addition of crude proteinbovine seminal plasma (1:2 proportion) had cause law motility, more proteins bindingwith sperm membrane will restricts movement of spermatozoa.

Keyword :

Crude protein seminal plasma, DNA Fragmentation, Necrosis, Ram Spermatozoa,

References :

  1. Alvarez, J.G., and Storey B.T. , (1995). Differential Incorporation of Fatty Acid Into and Peroxidative Loss of Fatty Acid from Phospholipids in Human Spermatozoa. . 42: 334-345 : Mol Repro Dee
  2. Asadpour, R., Alavi-Shoustari S.M., Rezaii S.A., and Ansari M.H., (2007). SDS-Polyacrilamide Gel Electrophoresis of Buffalo Bulls Seminal Plasma Protein and Their Relation with Semen Freezability.. 102: 308-313 : Animal Reprouction Science
  3. Edy, P., dan Taswin R.T., (2008). Pengaruh Penambahan Adenosin Triphosphat Kedalam Pengencer Semen terhadap Kualitas Spermatozoa Domba Ekor Gemuk. ... 10 (3): 151-156 : Animal Production


Archive Article

Cover Media Content

Volume : 10 / No. : 1 / Pub. : 2017-02
  1. Isolation and identification vibrio sp as vibriosis cause of agent at grouper fish (epinephelus fuscoguttatus) in banyuwangi regency
  2. Identification of species and stage of mite causes scabies by crusting severity on the rabbits
  3. Potency of curcumin on histopathological changes in the kidney of pregnant mice (mus musculus) exposed to cadmium
  4. Effect of vitamin c and e towards histopathological changes of duodenum in mice induced with borax
  5. The effects of crude protein bovine seminal plasma addition in skim milk and egg yolk diluent to motility, viability, dna fragmentation, and necrosis of ram spermatozoa after equilibration
  6. The effect of red fruit oil (pandanus conoideus lam.) on histopathology of follicle development in infertile mice modelling
  7. Prevalence and infection level of gastrointestinal worm nematode on long tailed macaque (macaca fascicularis) at taman wisata wendit malang regency
  8. Prevalence of distomatosis on various type of slaughtered cattle at penggaron semarang abattoir
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  11. Semen quality, hsp 70 expression and dna damage of post thawing limousine bull spermatozoa after cooled at 5 °c
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  15. The effect of mimosa pudica root extract to histopathological representation of rattus norvegicus liver injected with naja sputatrix venom
  16. Androgen dihydrotestosterone and its role in male reproductive system