Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 2 / No. 2 / Published : 2009-07

Order : 15, and page :169 - 174

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Original Article :

The effects of trehalose level in tris based diluter to cross breed etawah goat spermatozoa quality after storage at room temperature

Author :

  1. E. Sugiharta *1
  2. N. Isnaini*2
  1. Alumnus Fakultas Peternakan Universitas Brawijaya Malang
  2. Staf Pengajar Fakultas Peternakan Universitas Brawijaya Malang

Abstract :

This research was conducted to evaluate the effects of trehalose level in tris based diluter to crossbreed Etawah goat spermatozoa quality after storage at room temperature. Semen obtained from five buckscross breed Etawah goat with minimum requirements: individual motility 70% and mass motility 2+. This method of this research was experimental laboratory method by four treatments trehalosae level 0, 1, 2 and 3%. The design was Completely Randomized Design (CRD) and if there are differences, it would be continued by Duncan Multiple Range Test. The results showed that trehalose level was hight significant different (P<0.01) for percentage of individual motility on 2 hours at room temperature storage, and significant different (P<0.05) for percentage of live spermatozoa on 6 hours at room temperature storage. It can be concluded that 2% trehalose level have the best percentage of motility on 2 hours room temperature storage and 3% trehalose level have the best percentage of spermatozoa life on after 6 hours at room temperature storage. In tris based diluter trehalose was not effective for spermatozoa storage in room temperature.

Keyword :

trehalose, spermatozoa quality, tris aminomethane, Room temperature, Cross Breed Etawah Goat,

References :

Banaroudj,N., D.H. Lee and A.L. Goldberg,(2001) Trehalose Accumulation During Cellular Stress Protects Cells and Cellular Proteins from Gamage by Oxygen radicals . : J.Biol.Chem.

Elbein, A.D., Y.T. Pan, I. Pastuszak and D. Caroll.,(2003) New Insights on Trehalose: a Multifunctional Molecule . : Glycobiology

Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2009-07
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  2. Characterization Of Bacterial Surface Protein On Staphylococcus Aureus And Staphylococcus Epidermidis From Milk Cows With Mastitis
  3. The Sensitivity Of Coagulase Slide Test For Detection Of Staphylococcus Aureus On Bovine Mastitis Cases
  4. The Motility And Viability Of Entok (cairina Moschata) Spermatozoa In Diluter Of Skim Milk-fructosa
  5. The Effect Of Cryoprotectan Combination Dimethyl Sulfoxyde (dmso) And Glycerol At Frozen Semen Extender Of Cattle For Spermatozoa Quality Pasca Thawing
  6. Control Intra Vaginal Device Release (cidr) In Artificial Insemination Program Of Beef Catlle In Banyuwangi Region
  7. Water Injection On Waer Needling Acupuncture Analgesic Effect To Zusanli Acupoint Of White Rats (rattus Novegicus)
  8. Test Of Pharmacological Effect Of Eucheuma Spinosum Extract For Decreasing Blood Glucose Levels On Mice (rattus Novergicus) Induced Multi-low Dose Streptozotocin (mld Stz)
  9. Male Infertility: Sperm Dna Damage, Reactive Oxygen Species (ros) And The Key Role Of Protamine Molecule
  10. The Influence Of Lps ( Iipopolysaccharide) To Maltase Activity Isolated From Rattus Norvegicus Jejunum
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  12. Introduction Polyclonal Antibody Tyrosine Kinase As Alternative Immunocontraception Method Against Fertility Level Of Male Mice
  13. Bovine Oocytes In Vitro Maturation In Tcm 199 Supplemented By Bovine Hypophisis Extract
  14. The Effects Of Trehalose Level In Tris Based Diluter To Cross Breed Etawah Goat Spermatozoa Quality After Storage At Room Temperature
  15. The Fertility Acceptor Of Dairy Cow Artificial Insemination In Kpsp Setia Kawan Nongkojajar Tutur District Pasuruan Regency
  16. The Influence Of Bacteriocin Which Was Produced By Lactic Acid Bacteria Of Pediococcus Pentosaceus As A Teat Dip Substance Against Sub Clinical Mastitis