UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 2 / Published : 2011-07

Order : 4, and page :101 - 104

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Original Article :

The effects of various concentration propanediol cryoprotectan at vitrification process to viability of mice embryos post thawing

Author :

  1. Widjiati*1
  2. Ratri, A.W*2
  3. Arifin, Zainal*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This experiment was doing to know the effects concentration of cryoprotectant at vitrification on viability of mice’s embryos. Embryos were exposed to a different concentration of propanediol cryoprotectant, that is concentration 10%, 20% and 30%. Embryos were kept in mini straw and then plunged into liquid nitrogen rapidly. After 2 week, the frozen embryos were thawed in a 30°C water bath and be washed twice in 0,5 M Sucrose. Viability of embryos was examined used inverted microscope. The result showed there have a significant differences (p < 0,05) for embryos survival among treatment of propanediol 10% (46,95 %), propanediol 20%(51,73 %) and propanediol 30% (54,94 %). A various concentration of cryoprotectant was give a different result atembryos viability. The conclusion of this experiment there is a real difference (p < 0,05) at a various concentration of propanediol cryoprotectant with a good result give in propanediol 30% (54,94 %).

Keyword :

Vitrification, Cryoprotectant, Concentration, Viability, Embryos,


References :

Dike, I.P and S Palaniswamy,(2011) Vitrification of sheep oocyte using propylene glycol and its effect on the viability and fertilizability and chromosome patterns United States : International J. Current Research. 3(4) : 69 – 73.

Cseh. S., J. Corselli, S.L. Nehlsen-Cannarella, L.L. Bailey and Szalay,(1997) The effect of quick freezing in ethylen glycol on morphologycal survival and in vitro development of mouse embryos frozen at different preimpletation stages United States : Theriogenology. 48 : 43 – 50.

Vajta, G,(2000) Vitrification of the oocyte and embryos in domestic animals United States : Anim Reprod. Sci. 61 : 357 – 364.





Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2011-07
  1. The Effect Of Tyrosine Kinases Addition Into The Egg Yolk Skim And Egg Yolk Tris Extender The Motility And Live Spermatozoa Percentages Of The Holstein Friesian Post Thawing
  2. Avian Influenza H5 Virus Excretion Post Challenge Test Of Chickens Immunized With Avian Influenza (h5n1) Virus Indonesian Strain And Commercial Heterologous Vaccine
  3. Reepithelialization And Ck 16 Expression On Superficial-thickness Wound With Topical Hyaluronate And Freeze-dried Amniotic Membrane Treatment
  4. The Effects Of Various Concentration Propanediol Cryoprotectan At Vitrification Process To Viability Of Mice Embryos Post Thawing
  5. Activity Test Of Gynura Procumbens Leaves Extract As Antiangiogenic On Chick Embryo Chorioallantoic Membrane Induced By Basic Fibroblast Growth Factor (bfgf)
  6. Efect Of Centrifugation Of Goat Semen To Free Radical Concentrate On Washing Medium
  7. Isolation Identification And Sensitivity Test Of Bacillus Subtilis From Shrimp Culture Ponds To Antimicrobial Agents
  8. The Differences In Several Parameters Of The Femur Bone Related To The Biomechanics Of The Bucks And Doe
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  10. Antibody Response And Protectivity In Chickens After Vaccination With Nd Lv12 Active Vaccine
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  14. Antibacterial Potency Of Honey And Noni Juice (morinda Citrifolia L.) To The Number Of Staphylococcus Aureus On Chicken Carcasses
  15. Mengukur Kadar Protein Fertility Associated Antigen (faa) Pada Semen Sapi Menggunakan Qubit Fluorometer

Information VETMED