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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 1 / Published : 2014-02

TOC : 15, and page :93 - 99

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Original Article :

The giving of anti- toxoplasma gondii membran immunoglobulin y before infection reduce apoptotic index liver mice

Author :

  1. Heni Puspitasari*1
  2. Lucia Tri Suwanti*2
  3. Suwarno*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The study was aimed to determine the effect of administering anti-T. gondii membran IgY against liver damage (Apoptotic index) and the effectiveness of the antibody delivery time. This research was a laboratory experimental with five treatments and five replicates each treatment using female mice (Mus musculus) as animal models. The treatment groups consisted of a P0 group (not infected), P1 group (infected), P2 group (anti-T. gondii membran IgY giving one day before infection), P3 group (anti-T. gondii mmbran IgY giving together with infection) and P4 group (anti-T. gondii membran IgY giving two days after infection), at a dose of anti-T. gondii membrane IgY as many as 75 ug/head and infectious dose by 10 tacyzoite/head, four days after infection mice were sacrificed, examined and counting apoptosis index in histopathological liver using Apoptotic kit ApopTag®Plus Peroxidase In Situ (Chemicon®International, S7101). Percentage of apoptosis index liver each treatment is P0 was 3,64%, P1 was 12.98%, 6,06% for P2, 7,73% for P3 and 10,49% for P4. Thus, it can be concluded that administration of anti-T. gondii membran IgY can reduce the liver cell apoptotic index and it was greatest when given before and simultaneously with infection.

Keyword :

Toxoplasma gondii, Immunoglobulin Y, Liver damage.,

References :

  1. Begum-Haque, S., A. Haque and L.H. Kasper, (2009). Apoptosis in Toxoplasma gondii Activated T cells: The Role of IFNγ in Enhanced Alteration of Bcl-2 Expression and Mitochondrial Membrane Potential. . : Microb Pathog. 47 (5): 281-288
  2. Mordue, D.G., F. Monroy., M.L. Regina.,C.A. Dinarello and L.D. Sibley, (2001). Acute Toxoplasmosis Leads to Lethal Overproduction of Th1 Cytokines.. . : The American Association of Immunologists. 167: 4574-4584
  3. Nomura, K., H. Imai, T. Koumura, T.Koebayashi and Y. Nakagawa, (2000). Mithochondrial Phospholipid hydroperoxide glutathione peroxidase inhibists the release of cytocrome c from mithichondrial by suppressing the peroxidation of cardiolipin in hypoglycaemia induced apoptosis. . : Biochem J. 351: 183-193


Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2014-02
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  2. Effect of infusum salam leaves (eugenia polyantha) on blood glucose level on the rat (rattus norwegicus) in alloxan induced
  3. Potential of phytase ezymes from rumen bacteria on sem analysis structural changes of rice bran as broiler feed
  4. The comparison of morphology between bovine oocyte vitrification before and after in vitro maturation
  5. The effect of exposing pregnant rat (rattus novergicus) to particulate matter on congenital disorder and resorption of embryos
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  15. The giving of anti- toxoplasma gondii membran immunoglobulin y before infection reduce apoptotic index liver mice