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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 1 / Published : 2014-02

TOC : 15, and page :93 - 99

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Original Article :

The giving of anti- toxoplasma gondii membran immunoglobulin y before infection reduce apoptotic index liver mice

Author :

  1. Heni Puspitasari*1
  2. Lucia Tri Suwanti*2
  3. Suwarno*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The study was aimed to determine the effect of administering anti-T. gondii membran IgY against liver damage (Apoptotic index) and the effectiveness of the antibody delivery time. This research was a laboratory experimental with five treatments and five replicates each treatment using female mice (Mus musculus) as animal models. The treatment groups consisted of a P0 group (not infected), P1 group (infected), P2 group (anti-T. gondii membran IgY giving one day before infection), P3 group (anti-T. gondii mmbran IgY giving together with infection) and P4 group (anti-T. gondii membran IgY giving two days after infection), at a dose of anti-T. gondii membrane IgY as many as 75 ug/head and infectious dose by 10 tacyzoite/head, four days after infection mice were sacrificed, examined and counting apoptosis index in histopathological liver using Apoptotic kit ApopTag®Plus Peroxidase In Situ (Chemicon®International, S7101). Percentage of apoptosis index liver each treatment is P0 was 3,64%, P1 was 12.98%, 6,06% for P2, 7,73% for P3 and 10,49% for P4. Thus, it can be concluded that administration of anti-T. gondii membran IgY can reduce the liver cell apoptotic index and it was greatest when given before and simultaneously with infection.

Keyword :

Toxoplasma gondii, Immunoglobulin Y, Liver damage.,


References :

  1. Begum-Haque, S., A. Haque and L.H. Kasper, (2009). Apoptosis in Toxoplasma gondii Activated T cells: The Role of IFNγ in Enhanced Alteration of Bcl-2 Expression and Mitochondrial Membrane Potential. . : Microb Pathog. 47 (5): 281-288
  2. Mordue, D.G., F. Monroy., M.L. Regina.,C.A. Dinarello and L.D. Sibley, (2001). Acute Toxoplasmosis Leads to Lethal Overproduction of Th1 Cytokines.. . : The American Association of Immunologists. 167: 4574-4584
  3. Nomura, K., H. Imai, T. Koumura, T.Koebayashi and Y. Nakagawa, (2000). Mithochondrial Phospholipid hydroperoxide glutathione peroxidase inhibists the release of cytocrome c from mithichondrial by suppressing the peroxidation of cardiolipin in hypoglycaemia induced apoptosis. . : Biochem J. 351: 183-193


   


Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2014-02
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  2. Effect of infusum salam leaves (eugenia polyantha) on blood glucose level on the rat (rattus norwegicus) in alloxan induced
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  4. The comparison of morphology between bovine oocyte vitrification before and after in vitro maturation
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  15. The giving of anti- toxoplasma gondii membran immunoglobulin y before infection reduce apoptotic index liver mice