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Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

TOC : 1, and page :100 - 105

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Original Article :

The influence of carbofuran exposure toward white pulp diameter of spleen of mice (mus musculus)

Author :

  1. Yohana Anggarasari*1
  2. Epy Muhammad Luqman*2
  3. Bambang Poernomo*3
  4. Didik Handijatno*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of the research was to observed the white pulp diameter of mice spleen histopathology through carbofuran exposured. Eighteen females Balb/C strain mice (Mus musculus) were aged 10 weeks and average body weights 30 grams has been used. The population of mice were divided into three groups i.e. P0, P1, and P2, each consist of six. Doses of each sample was 1 ml orally administered through gastric lavage, once a day, every morning, until day 10th. The P0 was given NaCl physiologic 0,9%, while both treatment groups were P1 and P2 given carbofuran doses 0,0208 and 0,0417 mg/kg of body weight, respectively. All of mices were sacrificed on day 12th, and followed through necropsy to observed the spleen. After that, the spleen histopathology was carried out in hematoxylin eosin (HE) stained. The spleen histopathology observation was carried out through light microscope at 200 times of magnification. The spleen histopathology examination was done to measure the average diameter of white pulp. The white pulp average was analyzed by F test of ANOVA with SPSS 16.0 for Windows program. The result showed that there was a significantly difference between both control and treatment groups (p<0,05). However, carbofuran exposure reduced the white pulp diameter of spleen of mice.

Keyword :

White Pulp Diameter, Carbofuran,

References :

  1. Brambilla, D., C. Mancuso, M.R. Scuderi, P. Bosco, G. Cantarella, L. Lempereur, G.D. Benedetto, S. P, (2008). The Role of Antioxidant Supplement in Immune System, Neoplastic, and Neurodegenerative Disorders: a Point of View for an Assessment of the Risk/Benefit Profile.. . : Nutr. J. 7: 29-38.
  2. Luqman, E.M., (2012). Pengaruh Pajanan Insektisida Karbofuran terhadap Aktivitas ROS, Ekspresi P53 dan Caspase 3 dan Kematian Sel Neuron Korteks Serebrum Embrional Mencit (Mus musculus). Surabaya : [Disertasi] Program Studi Ilmu Kedokteran Program Pasca Sarjana. Universitas Airlangga
  3. Powers, S.K and M. Jackson, (2008). Exercise-Induced Oxidative Stress: Cellular Mechanism and Impact on Muscle Force Production. . : Physiol. Rev. 88: 1243-1276


Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
  1. The influence of carbofuran exposure toward white pulp diameter of spleen of mice (mus musculus)
  2. Antibacterial activity of the supernatant of soil isolate bacillus subtilis against aeromonas hydrophila and staphylococcus aureus (in vitro)
  3. The potential of giving synbiotic in different ages of female broilers on histological of ileum
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