Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 2 / No. 2 / Published : 2009-07

Order : 10, and page :137 - 144

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Original Article :

The influence of lps ( iipopolysaccharide) to maltase activity isolated from rattus norvegicus jejunum

Author :

  1. Anna Roosdiana*1
  2. Aulanni’am*2
  3. Reza Ranuh*3
  4. Asti Rahmanita*4
  1. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya
  2. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya
  3. Fakultas Kedokteran Universitas Airlangga
  4. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya

Abstract :

Maltase is an enzyme which exists on brush border membrane especially in jejuna mucosa cells. The maltase functions to hydrolyze maltose become glucose molecule. Lower activity of maltase is caused by Gastroenteritis which has diarrhea symptom due to LPS induction. Because LPS is a component on the outer membrane of gram-negative bacteria, endotoxin, it can stimulate the production of ROS (Reactive Oxygen Species) to make inflammation. Rattus norvegicus was used as a model and it was treated some procedures, such as LPS induction with dose of 2.5 mg/kg weight, determination of maltase activity using Nelson Somogyi method, and it’s molecular weight and Hematoxylen-Eosin staining of jejunum. LPS induction can damage the jejuna mucosa cells which were showed by the histological pictures and the loss of some protein bands. The average of maltase activity isolated from Rattus norvegicus jejuna that has been induced by LPS and control were 19.05±0.03 Unit and 21.89±0.04 Unit respectively. The induction of LPS will decrease the maltase crude activity significantly.

Keyword :

LPS, Rattus norvegicus jejunum, maltase activity, ., .,

References :

Alikhani, M., Z. Alikhani, D.T. Graves. ,(2004) Apoptotic Effects of LPS on Fibroblasts are Indirectly Mediated through TNFR1 . : J Dent Res

Amiruddin, R,(2007) Current Issue Kematian Anak (Penyakit Diare) Makasar : Fakultas Kesehatan Masyarakat jurusan Epidemiologi Universitas Hasanuddin

Armitage, David,(2004) Rattus norvegicus Michigan, USA : Animal Diversity Web. University of Michigan of Zoology

Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2009-07
  1. Protein Digestibility Of Laying Hens Added Suplementation Probiotic-chlorella
  2. Characterization Of Bacterial Surface Protein On Staphylococcus Aureus And Staphylococcus Epidermidis From Milk Cows With Mastitis
  3. The Sensitivity Of Coagulase Slide Test For Detection Of Staphylococcus Aureus On Bovine Mastitis Cases
  4. The Motility And Viability Of Entok (cairina Moschata) Spermatozoa In Diluter Of Skim Milk-fructosa
  5. The Effect Of Cryoprotectan Combination Dimethyl Sulfoxyde (dmso) And Glycerol At Frozen Semen Extender Of Cattle For Spermatozoa Quality Pasca Thawing
  6. Control Intra Vaginal Device Release (cidr) In Artificial Insemination Program Of Beef Catlle In Banyuwangi Region
  7. Water Injection On Waer Needling Acupuncture Analgesic Effect To Zusanli Acupoint Of White Rats (rattus Novegicus)
  8. Test Of Pharmacological Effect Of Eucheuma Spinosum Extract For Decreasing Blood Glucose Levels On Mice (rattus Novergicus) Induced Multi-low Dose Streptozotocin (mld Stz)
  9. Male Infertility: Sperm Dna Damage, Reactive Oxygen Species (ros) And The Key Role Of Protamine Molecule
  10. The Influence Of Lps ( Iipopolysaccharide) To Maltase Activity Isolated From Rattus Norvegicus Jejunum
  11. Descriptive Study Of Effect Of Milk Replacer Feeding On Dromedary Camel Calf Body Growth Gain In Surabaya Zoo
  12. Introduction Polyclonal Antibody Tyrosine Kinase As Alternative Immunocontraception Method Against Fertility Level Of Male Mice
  13. Bovine Oocytes In Vitro Maturation In Tcm 199 Supplemented By Bovine Hypophisis Extract
  14. The Effects Of Trehalose Level In Tris Based Diluter To Cross Breed Etawah Goat Spermatozoa Quality After Storage At Room Temperature
  15. The Fertility Acceptor Of Dairy Cow Artificial Insemination In Kpsp Setia Kawan Nongkojajar Tutur District Pasuruan Regency
  16. The Influence Of Bacteriocin Which Was Produced By Lactic Acid Bacteria Of Pediococcus Pentosaceus As A Teat Dip Substance Against Sub Clinical Mastitis