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Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

TOC : 10, and page :313 - 320

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Original Article :

The potential of outer membrane protein brucella abortus local isolate as brucellosis vaccine development in indonesia

Author :

  1. Didik Handijatno*1
  2. Hani Plumeriastuti*2
  3. Wiwiek Tyasningsih*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Outer membrane protein (OMP) of local isolate Brucella abortus were studied by Polyacrylamide gel electrophoresis (SDS-PAGE) in an effort to amount of IgG concentration for development vaccines to protect against Brucellosis. Thirty local male rabbits aged 8 weeks were divided into three treatment that are P0 vaccinated with physiological saline (NaCl physiologis), P1 vaccinated with whole Brucella abortus S19 and P2 vaccinated with OMP of local isolate Brucella abortus. Booster vaccination performed twice at intervals for 2 weeks, and observations on the amount of IgG concentration by Elisa method in the first week, second week, third week and the fourth week after the last vaccination.The results showed that each treatment increased the amount of IgG concentration from the first week to the fourth week with an average of P0 is 5.46, 5.71, 5.73 and 5.86 μg / mL, P1 is 14.20, 14.75, 15.51 and 16.36 μg / mL, P2 is 15.43, 16.30, 16.95 and 18.90 μg / mL, and there are significant differences among the three treatments (p < 0.05). These results indicate that the OMP of local isolate Brucella abortus can be used as a vaccine candidate to protect againts Brucellosis.

Keyword :

OMP, local isolate Brucella abortus, IgG, Elisa,


References :

  1. Cha, SB., N. Rayamajhi, K. Mi-Lan, W.J. Lee, MK. Shin and H.S. Yoo, (2010). Comparative Study of Gamma Interferon Production in Mice immunized with Outer Membrane Proteins and Whole bacteria of Brucella abortus. 63: 49-51 : Jpn. J. Infect. Dis
  2. Khatun, M.M., M.A. Islam, B.K. Baek and S.I. Lee, (2009). Celluler and Humoral Immune Responses and Antigen Recognation in Sprague-Dawley Rats Experimentally Infected with Brucella abortus Biotype I. 4(6): 267-277 : Asian J.of Animal and Vet. Advances
  3. Noor, S.M, (2006). Brucellosis: Penyakit Zoonosis yang belum banyak dikenal di Indonesia. 16 (1) : 31 – 39 : Wartazoa


   


Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping viral nervous necrosis (vnn) in groupers in east java and west nusa tenggara
  2. Lactoferrin effect on biofilm formation of klebsiella pneumoniae isolated from mastitis cases of dairy cows
  3. Effect of insulin transferrin selenium (its) supplementation in maturation medium tcm-199 to bovine oocyte diameter
  4. Supplementation of insulin transferrin selenium on in vitro maturation cumulus oocyte complex (coc) against to mitogen activated protein kinase (mapk) expression and expanded cumulus mass
  5. Adaptation capability and antigen stability of newcastle disease virus local isolate on hela cell
  6. Comparison of antibody titre of anti brucella abortus using cft and ielisa to cattle with positive rbt
  7. Potency of apple vinegar as alternatif therapy against wound healing time of staphylococcus aureus infection on mice
  8. Suplementation of specific protein membrane spermatozoa in freezing medium to improvement efforts a succes cattle frozen semen quality post thawing
  9. Potention of alkaloid sambiloto (andrographis paniculata l) to total leukocytes and leukocytes differential count on rat after infected by salmonella typhimurium
  10. The potential of outer membrane protein brucella abortus local isolate as brucellosis vaccine development in indonesia
  11. The using of whole serum pmsg (pregnat mare serum gonadotropin) with sepadex extracted for increasing estrus induction of ettawa goat
  12. Toxicity test of singawalang (petiveria alliaceae) leaves extract to the hypertrophy of male white mice (mus musculus) cardiac muscle
  13. Effect of benzo(a)pyrene injection to the initiation of mammae cancer at mice