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Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 3 / Published : 2014-11

TOC : 12, and page :278 - 285

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Original Article :

The potential of soursop (annona muricata linn.) leaves extract as immunostimulantin rabbit pbmc

Author :

  1. Fedik Abdul Rantam*1
  2. Lucia Tri Suwanti*2
  3. Almaedawati Erina*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Mahasiswa Fakultas Kedokteran Hewan

Abstract :

The usage of plant-based tradisional medicine is getting popular because there is minimum risk to health unlike chemical or synthetic drugs. One of the alternative usage of herbal medicine as immunostimulant to increase immune system is by using soursop leaves (Annona muricata Linn.). Soursop leaves extract contains flavanoid from flavonol group which can improve activation and proliferation of T-cell. The aim of this research is to prove that soursop leaves ethanol extract with dosage 5 μg, 15 μg, 25 μg and 50 μg have immunostimulant potensial to increase the expression of T-cell and secretion IFNγ in PBMC White New Zealand rabbits. The result from immunocytochemistry for expression on White New Zealand rabbit PBMC culture shows the lowest dosage 5 μg of soursop leaves extract is able to stimulate the expression of T-cell (47.64±0.7021) which greatly differs from control group (K), comparative control Phylantus niruri extract (P0), and experimental group P2, P3 and P4 (p ≤ 0.05). The increased number of T-cell indicates that flavonol in soursop leaves helps to activation macrofag to release IL-12 and TNFα, cytokine TNFα induces NK cell to release IFNγ, IFNγ induces T-cell . The test for IFNγ amount secretion with ELISA method on White New Zealand rabbit PBMC culture show that at the lowest dosage 5 μg soursop leaves ethanol extract is able to increase the amount of secreted IFNγ (353.89±54.04) which greatly differs from control group (K), and experiment P2, P3 and P4 (p ≤ 0.05), but not significant difference from comparative control with Phylantus niruri extract (P0). The increase IFNγ secretion proves that glycoside flavonol in soursop leaves ethanol extract induces thymocyte to increase IFNγ transcription.

Keyword :

Soursop leaf, Annona muricata Linn, Imunostimulator, CD4, IFNγ,


References :

  1. Adewole, S.O. and A.C.M. Ezekiel, (2006). Morphological Changes and Hypoglycemic Effect of Annona muricata Linn. (Annonaceae) Leaf Aqueous Extract in Pancreatic B-Cells of Streptozotocin-Treated Diabetic Rats. . : African Journal of Biomed Research 9 : 173-187.
  2. Dewi, LK., W.Sri dan R. Muhaimin, (2013). Pengaruh Pemberian Ekstrak Etanol Daun Sirsak (Annona muricata Linn.) terhadap Peningkatan Jumlah Sel T CD4+ dan CD8+ pada Timus Mencit (Mus musculus). Malang : Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Brawijaya
  3. Rachmawati, E., K.Setyawati dan S. Hidayat, (2012). Efek Ekstrak Etanolik Daun Sirsak pada Proliferasi dan Apoptosis Sel Hela yang Dimediasi oleh p53. Malang : Jurnal Kedokteran Brawijaya (27) 28-33


   


Archive Article

Cover Media Content

Volume : 7 / No. : 3 / Pub. : 2014-11
  1. The accuracy of production equine chorionic gonadotropin (ecg) frozen dry from local horse for increasing the time onset of oestrus in madura cattle
  2. Effect of infusum kopi leaves (coffea robusta lindl.) on blood glucose level on the mice in alloxan induced
  3. Comparison of sperm quality after separation x and y chromosome between column albumin method and electric separating sperm (ess) method on ekor gemuk sheep
  4. Potential of vitamin e on spermatogenic cell number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  5. The comparison of maturation between bovine oocyte vitrification before and after in vitro maturation
  6. Potency of pediococcus pentosaceus protease on meat tenderness and histological
  7. Effect of vitamin e on decreased of the leydig cell necrosis number in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)
  8. The immunomodulator potency of combination of andrographis paniculata and echinacea purpurea on total and differential leucocytes count in mice with heat stress
  9. The effect of various egg duck concentration in skim milk as semen diluters of fat-tailed sheep for motility, viability and intact plasma membrane spermatozoa before freezing
  10. Antibacterial effect of mango’s leaves (mangifera indica) extract against staphylococcus aureus and escherichia coli
  11. Effectiveness of honey and the juice of noni (morinda citrifolia linn) as well as mixtures of the number of bacteria on chicken carcasses
  12. The potential of soursop (annona muricata linn.) leaves extract as immunostimulantin rabbit pbmc
  13. Crude protein and crude fiber in rice bran fermented by acidothermus cellulolyticus or aspergillus terreus wpl 109 or wpl 209
  14. Level of contamination of salmonella sp in white prawns (litopenaeus vannamei) sold at some wet and modern market in surabaya
  15. Potensi alkaloid achyranthes aspera linn terhadap ekspresi protein bax sel kanker payudara pada mencit (mus musculus) yang diinduksi benzopyrene