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Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 2 / Published : 2015-07

TOC : 7, and page :151 - 160

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Original Article :

The seminal plasma protein of cow sustain quality of beans goat spermatozoa after equilibration in freezing process

Author :

  1. Suherni Susilowati*1
  1. Dosen Fakultas Kedokteran Hewan

Abstract :

Until now freezing of bean goat semen post thawing is not satisfactory because of the low quality of spermatozoa obtained, it is because in seminal plasma of goats are trigliserol lipase enzyme that can lower sperm motility in milk diluent. The purpose of this study was to determine the quality of spermatozoa after addition of seminal plasma protein cow pea goat semen in a diluent skim milk after equilibration in the freezing process. Seminal plasma protein derived from cows cement shelters, then separated and the plasma protein further purified by sonication. Plasma protein cement is then coupled with beans goat semen that have been diluted with skim milk. This study consisted of three groups: P0 ie Control Group: Cement goat diluted with skim milk (without the addition of plasma proteins semen cow), Group P1: the addition of plasma proteins semen cow 1 part plus 1 part cement goat that has been diluted with skim milk and Group P2: the addition of seminal plasma protein cow's 2 parts to 1 part cement plus goat that has been diluted with skim milk. Then stored at a temperature of 5 ° C for 1 hour, called the equilibration time then examined motility, viability and integrity of the plasma membrane of the spermatozoa. The results showed that the addition of seminal plasma protein cow retain motility, viability and intact plasma membrane of goat spermatozoa in the diluent of skim milk.

Keyword :

Seminal plasma protein of cow, beans goat semen, motility, viability and intact plasma membrane,

References :

  1. Lessard, C.,S. Parent, P. LEclerc, J.L. Bailey and R. Sullivan, (2000). Cryopreservation Alters the Levels of the Bull Sperm Surface Protein P25b. 21: 700-707 : Journal Andrology
  2. Kasai, M., (1996). Simple and Efficient Methods for Vitrification of Mammalian Embryos. 42(1): 67-75 : Animal Reproduction Sciences
  3. Susilowati, S, (2007). Insulin Like Growth Factor-I Complex Plasma Seminalis Kambing Meningkatkan Kualitas Spermatozoa Hasil Sentrifugasi. 23 (1) : Media Kedokteran Hewan.


Archive Article

Cover Media Content

Volume : 8 / No. : 2 / Pub. : 2015-07
  1. The effect of acupuncture on sodium, potassium, and chloride blood plasma in thoroughbred horse after exercise
  2. The effects of insulin transferin selenium (its) supplementation on embryo freezing media at morula stage towards the percentage of embryonic development at blastula stage
  3. The liver histopathology of mice (mus musculus) cause exposure carbofuran insectiside
  4. Effect of mangosteen rind extract (garcinia mangostana l.) to necrosis alveolar type ii cell of lungonpregnant mice (mus musculus)exposured by cigarette smoke
  5. Histopathological appearance of gastrointestinal tract iv instar mosquito larvae of aedes aegypti after dipping with active compounds of permot leaf extract (passiflora foetida linn.) and its potential as bioinsecticide
  6. The effects of differences in the duration of the thawing of frozen semen to sperm quality peranakan ettawa goat
  7. The seminal plasma protein of cow sustain quality of beans goat spermatozoa after equilibration in freezing process
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  12. The effect of mangosteen peel extract (garcinia mangostana l.) to the number of placental trophoblast cells in mice (mus musculus) exposure to cigarette smoke
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