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Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 2 / Published : 2016-07

TOC : 4, and page :23 - 30

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Original Article :

Therapeutic effect of spirulinaplatensis extract on histopathological appearance of ethanol induced liver injury in rat (rattus norvegicus)

Author :

  1. Aisha Jenar Sukmaningbayu*1
  2. Sri Agus Sudjarwo*2
  3. Rudy Sukamto Setiabudi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Penelitian ini bertujuan untuk membuktikan potensi ekstrak Spirulina platensisdalam mengurangi kerusakan hati yang disebabkan oleh etanol di Tikus (Rattusnorvegicus). Dua puluh tikus jantan dibagi menjadi lima kelompok yang berisi masingmasingempat tikus. Terdapat dua kelompok kontrol dan tiga kelompok perlakuan, yangdiberikan 200, 400, dan 800 mg/kg BB ekstrak Spirulina platensis secara peroral. Selamatujuh hari pertama masing-masing kelompok diberi etanol 50% 10 ml/kg BB kecualiuntuk kelompok kontrol. Pada hari ke-8, pemberian etanol dihentikan dan dilanjutkandengan pemberian ekstrak Spirulina platensis untuk kelompok perlakuan dan solusi CMCNa 0,5% untuk kelompok kontrol selama total 14 hari. Pada hari ke-28, 24 jam setelahpemberian terakhir, evaluasi histopatologi dilakukan untuk menghitung nilai kerusakanhati berdasarkan degenerasi dan nekrosis hepatosit menggunakan pewarnaan HE denganpembesaran 400x. Data skor dianalisis menggunakan Kruskal Wallis dan Mann-Whitney.Hasil penelitian menunjukkan dosis ekstrak 200 mg/kg BB Spirulina platensis dapatmengurangi kerusakan hati yang diinsuksi ethanol pada Tikus (Rattus norvegicus) danmemberi perbedaan yang signifikan (p <0,05) antara kelompok perlakuan. Akan tetapi,ekstrak Spirulina dosis 400 dan 800 mg/kg BB menunjukkan hasil negatif dimanagambaran histopatologi menunjukkan banyak degenerasi dan nekrosis di beberapa daerah.

Keyword :

Spirulina platensis, ethanol, kerusakan hepar,


References :

  1. Amar AR and ME Maysa., (2010). Antiulcer effects of cinnamon and chamomile aqueous extracts in rat models. . vol. 6 pp.209– 216. : J Am Sci.
  2. Bhat VB and KM Madyastha., (2000). C-phycocyanin: A potent peroxyl radical scavenger in vivo and in vitro. . Vol.275 pp.20-25. : BiochemBiophys Res Commun.


   


Archive Article

Cover Media Content

Volume : 9 / No. : 2 / Pub. : 2016-07
  1. Therapy effect of (pandanus conoideus inn) on female mice (mus musculus) infertility model toward the folicle diameter size of pre antral and antral histopathological features
  2. The effects of insulin transferin selenium supplementation on mice (mus musculus) embryos vitrification media at morula stage towards the viability of blastomere cells using fluorescence techniques after warming
  3. Staphylococcus aureus contamination rate on beef slaughter results in pegirian surabaya slaughterhouse
  4. Response of antibodies formed as result of brucella suis infection in mice (mus musculus) with complement fixation test (cft)
  5. Therapeutic effect of spirulinaplatensis extract on histopathological appearance of ethanol induced liver injury in rat (rattus norvegicus)
  6. Detection gene coding capsule type a of pasteurella multocida from cases of fowl cholera by polymerase chain reaction
  7. The resistance of escherichia coli from pig suspect colibacillosis in a pig farm in loa duri ulu village to antibiotics
  8. The prevalence of bacteria escherichia coli on meat of horse slaughtered at tumpang abattoir in malang district east java
  9. Utilization of coconut shell liquid smoke as preservation in chicken intestine on the value of total plate count
  10. Effect of red fruit (pandanus conoideus lam.) oil to litter size and congenital defecton lead intoxicated pregnant mice (mus musculus)
  11. Sensitivity test of some antibiotic types on bacteria of endometritis in pig farm, sukapura, probolinggo
  12. Potential test of cucumber tree(averrhoa bilimbi) leaves suspension as alternative mackerel (scomberomorus sp) preservatives
  13. Potention of alkaloid sambiloto (andrographis paniculata l) to total leukocytes and leukocytes differential count on rat before infected by salmonella typhimurium
  14. Influence of red betel leaf’s extract (piper crocatum) to the histopatology image on rat’s skin which infected by staphylococcus aureus
  15. Characteristic morphology and biochemical of cellulolytic isolate cytophaga sp
  16. Red cell count (erythrocyte), hematocrit, and hemoglobin of catfish (clarias gariepinus) after infected by aeromonas hydrophila
  17. The effect of extract meniran (phyllanthus niruri linn.)to ileum histopathological changes in ratsinduced by alcohol