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Majalah Farmasi Airlangga (Airlangga Journal of Pharmacy)

ISSN : 0852-1050

Vol. 5 / No. 1 / Published : 2005-04

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Original Article :

Efek Antimalaria Ekstrak Sambiloto Terstandar (Parameter Kadar Aandrografolida) Pada Mencit Terinfeksi Plasmodium Berghei.

Efek Antimalaria Ekstrak Sambiloto Terstandar (Parameter Kadar Aandrografolida) Pada Mencit Terinfeksi Plasmodium Berghei

Author :

  1. Dwi Kusumawardhani --> Bagian Ilmu Bahan Alam Fakultas Farmasi Universitas Airlangga
  2. Aty Widyawaruyanti --> Bagian Ilmu Bahan Alam Fakultas Farmasi Universitas Airlangga
  3. Idha Kusumawati --> Bagian Ilmu Bahan Alam Fakultas Farmasi Universitas Airlangga

Abstract :

Andrographis paniculata Nees. which known as ‘sambiloto’ is used for antimalaria. Andrographolide is an main content of sambiloto which is believed to have antimalarial activity. The previous research had shown that sambiloto extract have antimalarial activity both in vitro and in vivo. But, there is no data for the effective dose of standardized sambiloto extract as antimalaria. In this research, the standardized extract of sambiloto was tested in vivo as antimalarial agent using Peter’s 4 day suppressive test. Mice (16-29 body weight) were infected with Plasmodium berghei intra peritoneally with suspension containing infected red blood cell taken from donor mice with parasitemia >20%, when parasite concentration in erythrocyte reached >1%, treatment with suspension extract was given for four consecutive days orally with doses 0.0005-100 mg andrographolide/kg mice body weight. Blood samples were taken to determine parasitemia level for seven days and compared to non treated and chloroquin treated subjects and ED50 was obtained by analyzing inhibition level in five days with probit analysis. The result showed that standardized sambiloto extract [(10.82±0.37)% andrographolide] has an ED50 value of 12.2223 mg extract equal to 1.3200 mg andrographolide/kg body weight against Plasmodium berghei.

Keyword :

Standardized sambiloto extract, andrographolide, antimalarial, Plasmodium berghei ,


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