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Detail Article

Clinical Pathology and Medical Laboratory

ISSN : 0854-4263

Vol. 12 / No. 2 / Published : 2006-03

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Original Article :

Evaluation of Immunochromatography Method for Determination of Immunoglobulin M and G Anti-dengue in Dengue Pediatric Patients)

Evaluasi Pemeriksaan Imunokromatografi untuk Mendeteksi Antibodi IgM dan IgG Demam Berdarah Dengue Anak

Author :

  1. Ety Retno Setyowati --> Bagian/Laboratorium Patologi Klinik FK Unair/RSU Dr Soetomo / -
  2. Aryati --> Bagian/Laboratorium Patologi Klinik FK Unair/RSU Dr Soetomo / -
  3. Prihatini --> Bagian/Laboratorium Patologi Klinik FK Unair/RSU Dr Soetomo / -
  4. M.Y. Probohoesodo --> Bagian/Laboratorium Patologi Klinik FK Unair/RSU Dr Soetomo / -

Abstract :

The gold standard diagnosis of DHF by RT-PCR needs a complex technology and is time consuming. Serological tests have been developed to detect IgM and IgG anti dengue to determine primary as well as secondary acute phase infection. IgM and IgG antidengue tests by immunochromatography have been used, due to a high diagnostic validity, also because they are simple, practicable, easy, rapid (15–30 minutes), can be used in a single serum sample. ELISA method has been used as a confirmation method. The aim of this study is to evaluate the immunochromatography method in detecting IgG and IgM anti dengue of DHF patients. The study was performed on 50 serum samples from patients of the ICU Department of Paediatrics Dr. Soetomo Hospital, Surabaya during July–August 2005 with dengue virus infection according to the 1997, WHO criterion and 27 serum samples from non dengue virus infection patients. ELISA method showed positive infection in 44 samples. Immunochromatography method showed positive infection in 43 samples, but was negative in 1 sample. Diagnostic sensitivity of Immunochromatography is 97.7% (43/44) and the diagnostic specificity is 92.6% (25/27). Immunochromatography method has a high diagnostic value in assisting the diagnosis of DHF.

Keyword :

DHF, immunochromatography, ELISA,


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Archive Article

Cover Media Content

Volume : 12 / No. : 2 / Pub. : 2006-03
  1. The Manual And Automatic Tests Results Of Erythrocyte Sedimentation Rate
  2. Drug Resistance Of Mycobacterium Tuberculosis
  3. Analysis Of Acid Fast Bacilli (afb) Findings And Concentrated Slides In Suspected Tuberculosis
  4. The Microorganism Pattern In The Vagina Of Pregnancy Women In Dr. Soetomo Hospital Surabaya
  5. Collection And The Limit Time Of Using Diapers Samples For Urin Related Culture
  6. Laboratoric Diagnosis Of Avian Influenzae (h5n1)
  7. The Habitualis Abortion In Antiphospholipid Syndrome
  8. Evaluation Of Immunochromatography Method For Determination Of Immunoglobulin M And G Anti-dengue In Dengue Pediatric Patients)
  9. Quality Control In Clinical Microbiology
  10. Old People And Diabetes Mellitus
  11. The Analysis Of Serum Osteocalcin Level On Osteopenic And Osteoporotic Subjects