Berkala Ilmiah Kimia Farmasi
ISSN 2302-8270
Vol. 3 / No. 1 / Published : 2014-06
Order : 5, and page :36 - 41
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Original Article :
Daya hambat kombinasi susu probiotik (lactobacillus acidophilus + lactobacillus bulgaricus) dan pasta tomat terhadap escherichia coli dan staphylococcus aureus
Author :
- Izza Rahmi Hidayah*1
- Noor Erma N. Sugijanto*2
- Isnaeni*3
- Mahasiswa Fakultas Farmasi
- Dosen Fakultas Farmasi
- Dosen Fakultas Farmasi
Abstract :
Lactic acid bacteria fermented in milk produces antibacterial compound such as bacteriocin, organic acid especially lactic acid (Klewicka, 2004). Tomato has antibacterial activity because of its phenolic compounds and lycopene (Krishna, 2013; Sung, 2007). This study evaluates the antibacterial activity of fermented milk of L. acidophilus, L. bulgaricus, L. acidophilus-L. bulgaricus, tomato paste and combination of fermented milk (L. acidophilus-L. bulgaricus) and tomato paste using agar diffusion methods. This study found that fermented milk of L. acidophilus and L. bulgaricus has antibacterial activity against Eschericia coli and Staphylococcus aureus. The inhibition zone of 8:2 ratio v/v of fermented milk (L. acidophilus-L. bulgaricus) against E. coli and S. aureus are 10,91 ± 0,03 mm and 11,73 ± 0,25 mm. Tomato paste in saline solution 20% m/v is active against E. coli with inhibition zone 9,18 ± 0,10 mm. In 9:1 ratio v/v of fermented milk (L. acidophilus-L. bulgaricus) and tomato paste in saline solution 20% m/v has inhibition zone 10,35 ± 0,22 mm for E. coli.
Keyword :
fermented milk, L. acidophilus, L. bulgaricus, tomato paste, antibacterial activity,
References :
Klewicka, E., Libudzisz, Z.,(2004) Antagonistic Activity of Lactobacillus acidophilus Bacteria Towards Selected Food-Contamining Bacteria Journal : Pol. J. Food Nutr. Sci
Krishna, J.M,(2013) Phytochemical Analysis and Antimicrobial Studies of Various Extracts of Tomato (Solanum lycopersicum L.). Journal : Sch. Acad. J. Biosci
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