UNIVERSITAS AIRLANGGA



Detail Article

Berkala Ilmiah Kimia Farmasi

ISSN 2302-8270

Vol. 3 / No. 2 / Published : 2014-11

Order : 5, and page :31 - 38

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Original Article :

Validasi metode spektrofotometri-vis pada penetapan kadar boraks di dalam bakso

Author :

  1. Sudjarwo*1
  2. Sri Poedjiarti*2
  3. Angerina M*3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Mahasiswa Fakultas Farmasi

Abstract :

Borax, in illictic additive substance, was added on certain food product as a preservatife and rubbery. Therefore, the determination of borax in food product such as meatball is very impotant in view of meatball is  a food product often consumed by the general public. UV-Vis Spectrophotometric method with curcumine 0,125% as a reagent and glacial acetic acid-sulphuric acid has been used for determination of borax in this research. Curcumin reagent select because sensitivity of the method and the reproducibility of the results obtained depend on the quality of the curcumin reagent, and on rigorous observance of the reaction conditions (temperature, time, reagent quantities). Used  glacial acetic acid-sulphuric acid  because in acid media, curcumin and boron form a violetred 2:1 complex called rosocyanin. The optimum result were obtained when 1,0 ml solution of  0,125% curcuumin and 1,0 ml condition concentrated sulphuric acid were added and the absorbance was measured after 70 minutes at 547 nm. The result shows linear regression was y = 1,3127x – 0,0994, r = 0,9690 > r table   (n = 5) is  0.878 and p = 0,007 (p< 0,01) and Vxo is 15,53%.  The detection limit were found to be 9,7.10-4 ppm and quantitation limit is 2,94. 10-3 ppm.  Respectively, the recovery percentage was 47,56% and the coefficient variation was 3,92%.The determination of borax in three meatball sample which were taken from a area Surabaya showed that sample is positive of borax and the concentration was 0,0205 ; 0,0151 ;  0,0210 (% w/w).      

Keyword :

Borax, meatball, validation, spectrophotometric UV-Vis, rosocyanin,


References :

Liu, Y.M. and Lee, K,(2009) Modification of The Curcumine Method Enabling Precise and Acurate Measurement of Seawater Boron Concentration Pohang : School of Envirotimental Science and Engineering. Pohang University of Science and Technology. Republic of Korea.





Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-11
  1. Pembuatan Pereaksi Cepat Untuk Deteksi Dini Cemaran Logam Berat Cadmium
  2. Docking Molekul Dan Sintesis Turunan Asam Benzoil Salisilat Tersubstitusi Klor Sebagai Penghambat Siklooksigenase-2
  3. Aktivitas Antibakteri Kombinasi Probiotik (bifidobacterium Bifidum Dan Lactobacillus Acidophilus) Dengan Infus Daun Jambu Biji (psidium Guajava)
  4. Purifikasi Parsial Enzim Fibrinolitik Tempe Kacang Koro (canavalia Ensiformis) Produk Fermentasi Rhizopus Oryzae Fncc 6078
  5. Validasi Metode Spektrofotometri-vis Pada Penetapan Kadar Boraks Di Dalam Bakso
  6. Upaya Untuk Menghasilkan 1-(4-bromobenzoyloxy)urea Sebagai Calon Obat Antikanker
  7. Sintesis Dan Uji In Silico Turunan Asam Fenilpropanoat Sebagai Anti Platelet

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