UNIVERSITAS AIRLANGGA



Detail Article

Berkala Ilmiah Kimia Farmasi

ISSN 2302-8270

Vol. 2 / No. 1 / Published : 2013-06

Order : 1, and page :1 - 8

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Original Article :

Validasi spektrofotometri visible untuk penentuan kadar formalin dalam daging ayam

Author :

  1. Sudjarwo*1
  2. Sri Poedjiarti*2
  3. Pramitasari A.R*3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Mahasiswa Fakultas Farmasi

Abstract :

Formalin is a commercial name of formaldehyde solution 35-40% in water. Formalin kills virus, bacteria, fungi and parasite and has found wide use as a disinfectant with a broad efficiency. Formalin is often used as a food preservative with concentration range from 1,88–413,89 ppm (mg/kg), although it is not permitted since formalin is toxic for human consumption. Therefore the requirement of a chemical reagent for the examination of formalin in food is very necessary. The aim of this research is validating Spectrophoto-metric method to detect formalin in chicken meat.This research is conducted to develop method to detect formalin in chicken meat by reaction with chromotropic acid in strongly acidic media producing violet-red colour followed by detection with Spectrophotometric method at 567,0 nm. The research shows that the method is able to detect formalin in chicken with limit of detection is 0,0058 ppm and limit of quantitation is 0,0192 ppm. The standard curve gives regression y = 0,5233x+0,0397, r value is 0,9998 (p<0,01). Precision study shows  Coefficient Variation 0,4039%. Recovery study shows (87,3934±1,0826)%. The samples of chicken collected from a traditional market in Surabaya are detected concentration formalin range from 74,7595-140,2042 ppm (µg/g; wet weight).   Keywords : validation,Spectrophotometric, formalin, chicken meat, chromotropic acid.

Keyword :

validation, Spectrophotometric, formalin, chicken meat, chromotropic acid,


References :

Mulja, M., Syahrani, A,(1989) Aplikasi Analisis Spektrofotometri UV-Vis Surabaya : Mephiso Grafika

Sakiara, K.A., Pezza, L. , Melios, C.B., Pezza, H.R., Moraes, M,(1999) Spectrophotometric Determination of Dipyrone in Pharmaceutical Preparations by Using Chromotropic Acid Journal : Farmaco Vol 54

Fagnani, E., Melios, C.B., Pezza, L., Pezza, H.R. ,(2003) Chromotropic acid/formaldehid reaction in strongly acidic media. The role of dissolved oxygen and replacement of concentrated sulphuric acid Journal : Talanta vol. 60





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2013-06
  1. Validasi Spektrofotometri Visible Untuk Penentuan Kadar Formalin Dalam Daging Ayam
  2. Validasi Metode Kromatografi Gas-spektrometri Massa Untuk Penetapan Kadar Residu Endosulfan Dalam Kubis
  3. Sintesis Dan Uji Aktivitas Antikanker Senyawa 1-(2-klorobenzoiloksi)urea Dan 1-(4-klorobenzoiloksi)urea
  4. Pengaruh Gugus Metoksi Posisi Orto (o) Dan Para (p) Pada Benzaldehida Terhadap Sintesis Turunan Khalkon Dengan Metode Kondensasi Aldol
  5. Modifikasi Struktur N-fenilurea Menjadi Senyawa Baru N-benzoilfenilurea Dan 4-fluorobenzoilfenilurea Serta Uji Aktivitasnya Sebagai Penekan Susunan Saraf Pusat
  6. Aktivitas Antinociceptiv Dan Uji In Silico Terhadap Cyclooxygenase Dari Asam P-metoksisinamat Dan Asam M-metoksisinamat
  7. Docking Dan Modifikasi Struktur Senyawa Baru Turunan Parasetamol

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