UNIVERSITAS AIRLANGGA



Detail Article

Journal Of Emergency

ISSN 2301-7864

Vol. 3 / No. 1 / Published : 2014-06

Order : 2, and page :10 - 13

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Original Article :

Effect of oral pomegranate (punica granatum) peel aqueous extract on macrophage, fibroblast and collagen thickness in burn wound healing

Author :

  1. Dr. Iswinarno Doso Saputro, dr, SpBP-RE (K)*1
  2. Lobredia Zarasade, dr., SpBP-RE(KKF)*2
  3. Almahitta Cintami Putri*3
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Mahasiswa Fakultas Kedokteran

Abstract :

Background: Burn wound healing is a complex process, which involves the process of inflammation, oxidative stress, and infection. Therefore it is costly both to stabilize the general condition of the body, wound care itself, to prevent and to treat complications. Breakthroughs is needed to solve this problem in an effective, safe, and affordable ways. One alternative of the breakthroughs is to use natural ingredients and Pomegranate is one of that ingredients which is widely studied today. Pomegranates have antioxidant effects, anti-inflammatory, anti-infective and anticarcinogenic due to its content of polyphenols and derivatives, such ellagic acid, punicalagin, etc. Pomegranate polyphenol content higher than other fruits and it’s peel contain polyphenols pomegranate highest among other parts. It is known that the aqueous extract of pomegranate peel orally can stimulate healing of incision wound and pomegranate peel extract topically effective for burn wound healing. Research in the effect of oral pomegranate (punicagranatum) peel aqueous extract on burn wound healing of white rats have not been investigated.   Objective: To proof the effect of oral pomegranate (punicagranatum) peel aqueous extract on macrophage, fibroblast, and collagen thickness in burn wound healing of white rats.   Methods: An experimental with post-test only control group. A total of 28 rats with deep burn wound, divided into four groups: two groups of rats which will be given pomegranate peel aqueous extract 50mg/kgbw/day orally examined at 3 (inflammatory phase) and 10 (proliferative phase) days, and two groups of rats which will not be given the aqueous extract of pomegranate peel oral, examined at 3 and 10 days too. Burn wound will undergo histologic examination with hematoxylin-eosin staining counted the number of macrophages, fibroblast density, and thickness of collagen. Data were analyzed with multivariate analysis of variance analysis (Manova).   Results: The number of macrophage at 3 days was similar to the control, but at 10 days control increased significantly (P<0,05). The number of fibroblast was significantly higher at 3 days (P<0,005) but at 10 days was simmilar to the control. The collagen thickness at 3 days increasedalthough not statistically significantto the control but at 10 days control decreased significantly (P<0,05).   Conclusion :Oral pomegranate (punicagranatum) peel aqueous extract doesn’t decrease macrophage but inhibit its raising, increases fibroblast significantly, and increases collagen thickness although not statistically significant.

Keyword :

aqueous extract of pomegranate peel, polyphenols, burn wound healing,


References :

Moenadjat Y,(2001) Luka Bakar Pengetahuan Klinis Praktis 1-100 : Fakultas Kedokteran Universitas Indonesia Jakarta





Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-06
  1. Role Of Keratinocyte Progenitor Adipose Derived Stem Cells In The Epithelialization Of Skin Wound Healing In Rabbits
  2. Effect Of Oral Pomegranate (punica Granatum) Peel Aqueous Extract On Macrophage, Fibroblast And Collagen Thickness In Burn Wound Healing
  3. The Effect Of Amnion Freeze-drying To Prevent Adhesion In Rabbit Tendon Achilles Healing
  4. The Comparation Of The Use Of Multifilament Polyglycolide And Polyglyconate (barbed Suture) In Small Intestine Anatomosis With The One Layer Continous Technique
  5. Intraperitoneal Adhesion Prevention With An Intramuscular Injection Of Diphenhydramine Perioperative
  6. Blood Pressure And Pulse Rate Of Severe Preeclampsia S Receiving Magnesium Sulfate And Undergoing Cesarean Section Under General Anesthesia
  7. Clinical And Laboratory Profiles Relationships Of Preeclampsia-eclampsia Patients With Maternal Mortality In The Dr. Soetomo Hospital Surabaya
  8. The Difference Of Internal Diameter Of The Ring On Reponible Lateral Inuinal Hernia With Incarserated Lateral Inguinal Hernia On Children

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