UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 2 / Published : 2010-04

Order : 2, and page :88 - 94

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Original Article :

45 kda fimbria protein of proteus mirabilis as hemagglutinin and adhesion protein

Author :

  1. Diana Chusna Mufida*1
  2. Enny Suswati*2
  3. Septa Surya Wahyudi*3
  4. Agung Kurniawan*4
  1. Laboratory of Microbiology Faculty of Medicine University of Jember
  2. Laboratory of Microbiology Faculty of Medicine University of Jember
  3. Laboratory of Clinical Pathology Faculty of Medicine University of Jember
  4. Laboratory of Anatomy Faculty of Medicine University of Jember

Abstract :

Proteus mirabilis is opportunistic and nosocomial pathogen that usually found in clinical specimen from patients with catheter. The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity of the protein as hemagglutinin and adhesion molecule. The aim of this study is to evaluate the role of 45 kDa fimbria protein from P. mirabilis. After identification, bacterial isolate of fimbria fraction 12,5 % SDS-PAGE were  used to isolate fimbria protein followed by hemagglutinin test and in vitro adhesion test. The study showed that the 45 kDa fimbria protein of P mirabilis was a hemagglutinin protein that could agglutinate mice erythrocytes, rabbit erythrocytes, and human group O erythrocytes. Hemaglutination test were negative on erythrocytes human blood group A,B, and AB. The 45 kDa fimbriae protein was also adhesion protein showed by its activity to adherence to rabbit vesica urinaria epithel receptor. The increase dose of 45 kDa fimbriae protein will decrease the amount of P mirabilis bacteria to adherence to rabbit vesica urinaria epithel (p< 0,05 ).

Keyword :

Proteus mirabilis, fimbriae, hemagglutinin protein, adhesion protein,


References :

Ehara, M, Ishibashi, M, Ichinose, Y, Iwanaga, M, Schimotori, S & Naito, T,(1986) Purification and partial characterisationmaf fimbriae of Vibrio cholera 0-1 - : Vaccine

Hutson, AM, Atmar, RL, Graham, DY & Ester, MK,(2002) Norwalk virus infection and disease is assosiated with ABO histoblood group type - : Infecton Disease

Martino, PD, Bertin, Y, Girardeu, JP, Livrelli, V, Jolly, B & Daefeuille-Michaud, A,(1995) Molecular characterization and adhesive properties of CF29K, an adhesin of Kleibsiella pneumonia strain involved in nosocomial infection - : Infection and Immunity





Archive Article

Cover Media Content

Volume : 46 / No. : 2 / Pub. : 2010-04
  1. Polymorphism Of Natural-resistance-associated Macrophage Protein 1 (nramp1) D543n Gene And Expression Of Nramp1 On Lung Tuberculosis Patients And Nurses In Surabaya
  2. 45 Kda Fimbria Protein Of Proteus Mirabilis As Hemagglutinin And Adhesion Protein
  3. Diagnostic Value Of Widal Slide Assay Using One Phage Type Local Antigen Compared With Four Phage Types Local Antigen In Typhoid Fever Patients In Surabaya
  4. Differences Of Tumor Necrosis Factor Alpha (tnf-α) Plasma Concentration In Malaria Patients With Anemia And Without Anemia
  5. Differences Of Epidermal Growth Factor (egf) Concentration Between Unpreserved And Preserved Amniotic Membrane
  6. Cytotoxicity Effect Of Curcuma Aeruginosa Extract On Fibroblast With Mtt Assay Method
  7. Correlation Between Blood Serotonin Level With Consciousness Level And Depression Symptoms In Moderate Brain Injury Patients
  8. Blood Serotonin Level With Depression Situation And Neurocognitive As A Reflection Of Neuron Condition In Six Months After Moderate Brain Injury
  9. Association Between Heart Disease In Pregnancy With Cardiac Events
  10. Endoscopic Finding Of Upper Gastrointesinal Bleeding At Dr Soetomo Hospital, Surabaya
  11. Correlation Between Tnf-α, Il-1β, Pge2 And Spla2 Levels With Severity Of Dengue Hemorrhagic Fever
  12. Case Report: Auditory Neuropathy In Dr. Soetomo Hospital

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