UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 49 / No. 3 / Published : 2013-07

Order : 4, and page :150 - 154

Related with : Scholar   Yahoo!   Bing

Original Article :

Asymptomatic malaria in trenggalek district, east java province as revealed by microscopic examination and single step pcr

Author :

  1. Heny Arwati*1
  2. Kusmartisnawati*2
  3. Poppy*3
  4. Yoes Prijatna Dachlan*4
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Pandean Sub-District Community Health Center (Puskesmas), Trenggalek District, East Java Province, Indonesia
  4. Dosen Fakultas Kedokteran

Abstract :

Trenggalek district is one of hypoendemic areas of malaria in East Java Province. Most of the malaria cases are imported fromendemic areas in outside of Java Island, as the destination of the seasonal migratory workers. When they return to Trenggalekdistrict usually they are infected with malaria. Blood examination has been done to the patients who have recovered from malariainfection in order to find out the asymptomatic malaria in this region. The examination of Giemsa-stained blood smear is a goldstandard method recommended by WHO. However, due to the very low parasitemia in asymptomatic malaria makes difficulty inmicroscopic examination, the molecular diagnosis by single step PCR in this research is aimed to confirm the results of microscopicexamination of asymptomatic malaria. The objective of this paper was to find out malaria asymptomatic in Dongko sub-district,Trenggalek district by microscopic examination and single step PCR methods. Blood samples were collected during May andSeptember 2012 from the patients who have recovered from malaria infection. The number of sample was according to the list ofpatients hospitalized in Pandean public health service (Puskesmas Pandean), Dongko sub-district, Pandean district, East Javaprovince. Microscopic diagnosis was done on Giemsa-stained thick and thin smears at 1000x magnification. Molecular diagnosiswas done by single step PCR method according to Patsoula et al (2002). During May and September 2012, asymptomatic malariahave been found, where of the 18 samples, 5 samples were positive by microscopic examination two for P. falciparum, two for P.vivax, one for mix of both species, and 13 samples were negative. Single step PCR diagnosed two samples P. falciparum, twosamples as P. vivax, 6 samples as mix infection and 6 as negative samples. In conclusion, single step PCR provides a sensitive toolfor detection extremely low level of parasitemia in asymptomatic malaria. Mass screening of asymptomatic malaria is needed to beperformed simultaneously in collaboration with research institute or research hospital prior to mapping the cases and earlymedication. The presence of asymptomatic malaria cases is a challenge for the management of malaria elimination program in anyendemic area including Trenggalek District. (FMI 2013;49:150-154)

Keyword :

Asymptomatic malaria, single step PCR, Trenggalek district,


References :

Alves SP, Gil LH, Marrelli MT, Ribola PE, Camargo, EP, Pereira LH,(2005) Asymptomatic carriers of Plasmodium Sp. As infection source for malaria vector mosquitoes in Brazilian Amazon - : Journal of Medical Entomology

Bousema JT, Gouagna LC, Drakeley CJ, Meutstege AM, Okech BA, Akim IN, Beier JC, Githure JI, Sauerwei,(2004) Plasmodium falciparum gametocyte carriage in symptomatic children in western Kenya - : Malaria Journal





Archive Article

Cover Media Content

Volume : 49 / No. : 3 / Pub. : 2013-07
  1. Pharmacodynamics Study Of Cholecalciferol To Glut4 Protein Translocation In Muscle Fiber Of Hyperglycemia Micewhich Inducted By Streptozotocin
  2. The Comparison Of Simvastatin And Atorvastatin Efficacy In Lowering Lipid Profile And Apolipoprotein-b Of Diabetic Dyslipidemia Patient
  3. Sero-epidemiological Study On Dengue Virus Infection In Four Indonesian Cities
  4. Asymptomatic Malaria In Trenggalek District, East Java Province As Revealed By Microscopic Examination And Single Step Pcr
  5. Correlations Between Nutritional Status Changes With Number Of Cd4 Cell Changes In Hiv/aids Patients
  6. Comparison Of Platelet Anti Aggregation Effect On Acute Thrombotic Stroke With Diabetes Melitus And Non-dibetes Melitus
  7. Analysis Of Antibiotic Use In Vap (ventilator-association Pneumonia) Patients
  8. Differentiation Of Chloroform Etching Time On Acrylic Resin Denture Teeth To Shear Bond Strength With Resin Composite
  9. Profile Of Mdr-tb Patients In Dots Outpatient Clinic, Dr. Soetomo Hospital, Surabaya, From January 2010 To March 2011
  10. The Using Of Arv Pattern To Patients On Hiv/aids In Upipi Dr. Soetomo Hospital, Surabaya, January - June 2011
  11. Pharmaceutical Care And Anti-hypertension Treatment At Trf-ht Patients Undergoing Regular Hemodialysis. A Study At Hemodialysis Room, Dr Haryoto Hospital, Lumajang, East Java, Indonesia
  12. Management Of Antenatal Hydronephrosis. A Report Of 2 Cases

Information index.php/JIPK/FMI