UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 40 / No. 3 / Published : 2004-07

Order : 4, and page :76 - 85

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Original Article :

Correlation between the methods of flowcytometry, manual light and fluorescence microscopes for cd4 cell count in normal adults

Author :

  1. Endang Retnowati*1
  2. Wira Santoso*2
  3. Nasronudin*3
  1. Department of Clinical Pathology Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Surabaya
  2. Department of Clinical Pathology Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Surabaya
  3. Department of Internal Medicine Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Surabaya

Abstract :

A laboratory study was conducted in order to assess the correlation and to compare between flowcytometry, manual light and fluorescence microscope methods used for enumeration of CD4 cell count. K3EDTA whole blood samples from 15 healthy volunteers were taken for determining CD4 cell count by flowcytometry, manual light and fluorescence microscope methods. The results of the manual light and fluorescence microscope methods showed a significant correlation to the flowcytometry method, r = 0.949 (p = 0.000) and r = 0.959 (p = 0.000) respectively. In addition, the manual light microscope method demonstrated a significant correlation to the manual fluorescence microscope method, r = 0.927 (p = 0.000). The comparison between the flowcytometry method, the manual light and fluorescence microscope methods was not significantly different, respectively p = 0.175 and 0.055. Furthermore, there was no significant difference between the manual light microscope and the fluorescence microscope, p = 0.768. In using the manual light and fluorescence microscope methods, the CD4 cell count were enumerated by three different laboratory technicians. The results showed a significant correlation between the manual light microscope and flowcytometry methods, r = 0.943 (p = 0.000), r = 0.943 (p = 0.000) and r = 0.935 (p = 0.000), respectively. Moreover, compared to the flowcytometry method the results were not significantly different, p = 0.167, 0.121 and 0.412, respectively. The manual fluorescence microscope method showed a significant correlation to the flowcytometry method, r = 0.961 (p = 0.000), r = 0.947 (p = 0.000) and 0.932 (p = 0.000), respectively. The comparison to the flowcytometry method showed that the results of the first and second technician were not significantly different p = 0.642 and 0.052, respectively, however, the result of the third technician was significantly different (p = 0.018). Based on the results of the data analysis obtained in this study, it can be concluded that the manual methods can be used to replace the flowcytometry method for the determination of CD4 cell count.

Keyword :

flowcytometry, manual light, fluorescence microscopes for CD4 cell count,


References :

Bird AG,(1995) Quantification of CD4-positive T Lymphocytes. in Karn (editor) HIV, Virology and Immunology A Practical Approach, Volume 1 New York : Oxford University Press

Horsburgh Jr, CR,(1994) Prognostic Indicators For Progression of HIV Disease. in Schochetman (eds) AIDS Testing, A Comprehensive Guide to Technical, Medical, Social, Legal and Management Issues. Second Edition New York : Springer - Verlag





Archive Article

Cover Media Content

Volume : 40 / No. : 3 / Pub. : 2004-07
  1. Editorial Vol 40 No 3 2004
  2. Opinion: International Ethical Guidelines For Biomedical Research Involving Human Subjects
  3. Polymerase Chain Reaction (pcr) For The Diagnosis Of Cervical Tuberculous Lymphadenitis
  4. Correlation Between The Methods Of Flowcytometry, Manual Light And Fluorescence Microscopes For Cd4 Cell Count In Normal Adults
  5. Iron Deficiency, Low Arterial Oxygen Saturation And High Hematocrit Level As A Major Micro-environmental Risk Factors In The Development Of Brain Abscess In Patients With Tetralogy Of Fallot
  6. The Effect Of Cellulose On Apoptosis Of Colon Epithelial Cells Of Balb/c Mice That Induced By 9,10-dimethyl-1,2-benz(a)anthracene
  7. Preserved Amniotic Membrane Graft After Bare Sclera Technique With Mitomycin C For Pterygium Surgery
  8. Pharmacodynamic Identification Of Antiseizure Effect Of Ligustrosid Glycoside (a Cns Active Substance) Isolated From Fraxinus Griffithii Clarke On Mice
  9. The Modification Of Osce To Replace The Oral Examination In The Department Of Otorhinolaryngology, Airlangga University School Of Medicine
  10. Body Surface Area (bsa) As The Predictor Of Renal Parenchymal Volume In Healthy Adult
  11. Efficacy Of Ethyl Alcohol Glycerin 69% Handrub In Neonatal Ward Dr. Soetomo Hospital. A Randomised Controlled Trial
  12. Review Article And Clinical Experience: Recent Advances In Diabetes Treatment Options (cardioprotective Novel Oha And Insulin Glargine)
  13. Impacted Button Battery In The Nasal Cavity

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