UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 50 / No. 3 / Published : 2014-07

Order : 2, and page :139 - 142

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Original Article :

Detection of gyra gene mutation region in fluoroquinolone resistant mycobacterium tuberculosis isolate from surabaya indonesia

Author :

  1. Deby Kusumaningrum*1
  2. Ni Made Mertaniasih*2
  3. Nurul Wiqoyah*3
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Fluoroquinolones are an important class of antibiotics used in the management of bacterial diseases and drug resistancetuberculosis. This study aimed to detect gyrA gene mutations regions as the mechanism of fluoroquinolone resistance inMycobacterium tuberculosis by sequencing of the quinolone resistance determining region (QRDR) of gyrA, one of the genesencoding DNA gyrase. Mutations affecting amino acids at positions 74, 90, 91 and 94 have been most commonly associated withresistance. Drug susceptibility testing of Mycobacterium tuberculosis was performed by the culture method of proportion, includingsusceptibility to ofloxacine. DNA was extracted by boiling method. Nucleic acid was amplified by PCR, targeting the region of gyrAusing primers gyrA-F ( 5’CAG CTA CAT CGA CTA TGC GA 3’) andgyrA-R (GGG CTT CGG TGT ACC TCA T) and sequenced withABI 377 sequencer. Sequences were analysed to identify mutations associated with fluoroquinolone resistance. There were 5ofloxacine resistant Mycobacterium tuberculosis isolates which were PCR processed for gyrA. The amplicons product were 320 bpfrom five these isolates. No mutations were identified in gyrA specifically at sites corresponding with codons 90, 91, and 94, but all 5isolates had the mutations S95T. These findings suggest that the gyrA mutation, S95T is suspected as the polymorphism influoroquinolone resistant Mycobacterium tuberculosis. However, further investigation is required to identify the mechanism offluoroquinolone resistance in Mycobacterium tuberculosis. (FMI 2014;50:139-142)

Keyword :

fluoroquinolone resistance, Mycobacterium tuberculosis, gyrA, gene mutation,


References :

Chang KC, Yew WW, Chan RC,(2010) Rapid assays for fluoroquinolone resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis 65, 1551-1561 : Journal of Antimicrobial Chemotherapy

Aubry A, Veziris N, Cambau E, Truffot-Pernot C, Jarlier V, Fisher LM,(2006) Novel gyrase mutations in quinolone-resistant and -hypersusceptible clinical isolates of Mycobacterium tuberculosis: functional analysis of mutant enzymes 50, 104-112 : Antimicrob Agents Chemother





Archive Article

Cover Media Content

Volume : 50 / No. : 3 / Pub. : 2014-07
  1. Heavy Metal Contamination May Have A Role In Negative Correlation Between Seafood Consumption And Visuo-motor Development Of 1-3 Year-old Children In Subdistrict Bulak, Surabaya
  2. Detection Of Gyra Gene Mutation Region In Fluoroquinolone Resistant Mycobacterium Tuberculosis Isolate From Surabaya Indonesia
  3. Intravenous Vitamin C Administration Decreases Nitric Oxide Serum Level In Severe Burn Injury
  4. The Overview Of Macrophage, Mast Cell, And Lymphocyte Activity In The Efficacy Distinction Of Pain Reduction For Dry And Wet Cupping Therapies
  5. Effectiveness Of Fosfomycin On Prevention Of Surgical Site Infection Of Closed Brain Injury Patients After Craniotomy In Emergency Unit Dr. Soetomo Hospital
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  7. Electric Shock Increases Vcam And Mcp In Mice Endothelial Blood Vessel
  8. Magnesium And Potassium Parenteral Supplementation Does Not Produce Significant Effect On Magnesium And Potassium Serum Level Of Cervical Cancer Patients Receiving Cisplatin Chemotherapy
  9. Effect Of G-csf On Anc Level In All (acute Lymphoblastic Leukemia) Patient With Neutropenia And Febrile Neutropenia During Chemotherapy
  10. Comparative Effectiveness Of Paracetamol And Metamizol As Antipyretics In Patients With Moderate And Severe Brain Injury In Dr. Soetomo Hospital, Surabaya
  11. Analysis Of Complicated Factors Affecting Therapy Effectiveness In Urinary Tract Infections
  12. In Infant With Acute Diarrhea, Nutritional Status Has Correlation With Dehydration Degree, But Not With Mother’s Education And Breastfeeding
  13. The Use Of Antibiotics In Patients With Urinary Tract Infection

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